In purple carrots, anthocyanin pigmentation can be expressed in the entire root, or it can display tissue specific-patterns. Within the phloem, purple pigmentation can be found in the outer phloem (OP) (also called the cortex) and inner phloem (IP), or it can be confined exclusively to the OP. In this work, the genetic control underlying tissue-specific anthocyanin pigmentation in the carrot root OP and IP tissues was investigated by means of linkage mapping and transcriptome (RNA-seq) and phylogenetic analyses; followed by gene expression (RT-qPCR) evaluations in two genetic backgrounds, an F2 population (3242) and the inbred B7262. Genetic mapping of ‘root outer phloem anthocyanin pigmentation’ (ROPAP) and inner phloem pigmentation (RIPAP) revealed colocalization of ROPAP with the P1 and P3 genomic regions previously known to condition pigmentation in different genetic stocks, whereas RIPAP co-localized with P3 only. Transcriptome analysis of purple OP (POP) vs. non-purple IP (NPIP) tissues, along with linkage and phylogenetic data, allowed an initial identification of 28 candidate genes, 19 of which were further evaluated by RT-qPCR in independent root samples of 3242 and B7262, revealing 15 genes consistently upregulated in the POP in both genetic backgrounds, and two genes upregulated in the POP in specific backgrounds. These include seven transcription factors, seven anthocyanin structural genes, and two genes involved in cellular transport. Altogether, our results point at DcMYB7, DcMYB113, and a MADS-box (DCAR_010757) as the main candidate genes conditioning ROPAP in 3242, whereas DcMYB7 and MADS-box condition RIPAP in this background. In 7262, DcMYB113 conditions ROPAP.

En Argentina la especie arbórea Calophyllum brasiliense Cambess (Calophyllaceae) constituye poblaciones en los bordes de esteros y en las selvas higrófilas del río Paraná. Las poblaciones de la margen del río forman parte de ecosistemas fuertemente fragmentados por su asociación a las cotas de inundación del embalse Yacyretá, lo cual las ha llevado a reducir drásticamente su tamaño al punto de encontrarse en riesgo de extinguirse localmente. Uno de los efectos de la fragmentación en las especies vegetales es la producción de semillas en menor cantidad, calidad o ambas. El valor ecológico, medicinal, económico y social de la especie y el estado de sus poblaciones suscitaron interés en conservarla. Una de las estrategias implementadas fue el almacenamiento de sus semillas, las cuales son recalcitrantes. Los objetivos del presente trabajo fueron evaluar tres poblaciones en cuanto a su potencialidad de producir semillas capaces de generar individuos y evaluar la calidad fisiológica en relación al momento de cosecha y al tiempo de almacenamiento bajo cuatro modos de almacenamiento. La población de Puerto Valle produjo semillas de buena calidad que conservaron la viabilidad durante treinta y un semanas cuando se almacenaron con endocarpo, en frascos de vidrio.

The present study characterized a genetically and phenotypically diverse collection of 27 purple and two non-purple (one orange and one yellow) carrot accessions for concentration of root anthocyanins, phenolics, and carotenoids, and antioxidant capacity estimated by four different methods (ORAC, DPPH, ABTS, FRAP), in a partially replicated experimental design comprising data from two growing seasons (2018 and 2019). Broad and significant (p < 0.0001) variation was found among the accessions for all the traits. Acylated anthocyanins (AA) predominated over non-acylated anthocyanins (NAA) in all the accessions and years analyzed, with AA accounting for 55.5–100% of the total anthocyanin content (TAC). Anthocyanins acylated with ferulic acid and coumaric acid were the most abundant carrot anthocyanins. In general, black or solid purple carrots had the greatest TAC and total phenolic content (TPC), and the strongest antioxidant capacities, measured by all methods. Antioxidant capacity, estimated by all methods, was significantly, positively, and moderately-to-strongly correlated with the content of all individual anthocyanins pigments, TAC, and TPC, in both years (r = 0.59–0.90, p < 0.0001), but not with the carotenoid pigments lutein and β-carotene; suggesting that anthocyanins and other phenolics, but not carotenoids, are major contributors of the antioxidant capacity in purple carrots. We identified accessions with high concentration of chemically stable AA, with potential value for the production of food dyes, and accessions with relatively high content of bioavailable NAA that can be selected for increased nutraceutical value (e.g., for fresh consumption).

En este artículo, Sofía Carvajal, responsable de Desarrollo de Capacidades y Activación DATA en Movistar+, e invitada experta de la asignatura Investigación, estrategia y planificación de medios del máster universitario de Estrategia y Creatividad en Publicidad, explica los entresijos de la apuesta de Movistar+ por el nuevo modelo publicitario Addressable TV. Con esta solución publicitaria, basada en data de calidad prémium, la plataforma puede llegar de forma personalizada a sus 10 millones de abonados.