Aim: The aim of this study was to investigate the effects of integrated (INT), organic (ORG) and biodynamic (BD) management systems with similar C- and N-inputs on soil microbiology in a viticultural long-term field trial.Methods and results: Within the systems comparison, soil samples were taken 10 years after conversion, throughout the growing season. To gather information about microbial community structure, the activity of five soil enzymes was measured, and phospholipid fatty acids (PLFA) and neutral lipids fatty acids (NLFA) profiles were analysed accompanied by comprehensive soil analysis. pH associated with BD was significantly higher compared to INT soil. Copper and N-min values in INT were significantly lower compared to the organic systems. BD and ORG were characterised by a higher b-D-glucosidase and urease activity and a higher abundance of fungi and bacteria. INT had larger quantities of mycorrhizae indicator NLFAs.Significance and impact: Results from this study contribute to a better understanding of the microbial community structure and nutrient cycling under organic and biodynamic viticulture.