The study analyzed the relationship between promotion and discipline of employees in private universities in Uganda. It involved 385 respondents from seven private chartered and accredited universities. Data was collected using a self-administered questionnaire whose validity and reliability was confirmed through Factor Analysis and Cronbach Alpha test. Descriptive analysis involved the use of means (Averages), while Pearson Linear Correlation Coefficient was used to test the hypothesis. The results revealed that promotion was a positive significant determinant of discipline of employees. In conclusion, promotion was an important element in that it influenced the discipline of employees in private universities. It was recommended that managers of Organizations such as private universities should put emphasis on their promotion strategies which will influence the discipline of their employees and thus their rentation.