AIM OF THE STUDY:The present research attempts to assess the occurred linear changes of additional silicone impressions after utilizing different disinfection materials. MATERIALS AND METHODS:In the present study, a set of twenty-four impressions of a standard reference model were made using Polyvinyl siloxane impression material. The samples were split to form three groups (𝑤𝑤ℎ𝑒𝑒𝑒𝑒𝑒𝑒 𝑛𝑛 = 8) according to disinfection material used; sodium hypochlorite (SH), ozonated water (OW), and a control group (C). Group SH and Group OW were immersed at room temperature for up to 10 minutes. The prepared impressions were investigated before and after the disinfection procedure utilizing Dental Cone beam Computed Tomography (CBCT). Linear measurements were accurately determined between four reference points through the scanned digital models. After disinfection, the dimensional changes were calculated, and statistical analysis was conducted where the p-value was set as 𝑝𝑝 < 0.05. RESULTS: Each linear measurement for the three groups was analyzed using paired measurements before and after the disinfection process. The computed values prior to and following disinfection showed a significant difference when comparing the study groups as (𝑝𝑝 < 0.05). Moreover, the observed significant differences among the study groups were confirmed by applying one-way ANOVA analysis to assess the variance for each measurement. CONCLUSIONS: The dimensional accuracy of polyvinyl siloxane impression material performed well after using different disinfectant. Impressions were totally immersed in ozonated water showed better stability than impressions were totally immersed in NaOCl.