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RESUMO A Moringa oleifera Lam. (moringa) é uma espécie vegetal com grande potencial de uso nos setores alimentício, medicinal e forrageiro, no processo de clareamento e descontaminação de água, produção de biocombustível, dentre outros. Técnicas de produção de mudas com uso de resíduos regionais são apropriadas para a introdução desta espécie em comunidades carentes. O objetivo deste trabalho foi avaliar as respostas nutricionais e de crescimento de mudas de moringa, cultivadas em substratos com diferentes concentrações de fibra da casca de coco verde (FCV), associadas a composto orgânico (CO). O delineamento experimental adotado foi em blocos casualizados. Foram testados dois compostos orgânicos: o composto de lixo urbano (CLU) e o vermicomposto (VC) associados a FCV em várias proporções volumétricas (CO:FCV): 0:100; 25:75; 50:50; 75:25 a 100:0%. Após 51 dias de cultivo, foi observado que o aumento da concentração de FCV em relação ao composto orgânico reduziu a altura, a massa da matéria seca, o Índice de Qualidade de Dickson e os conteúdos de N, P, K, Ca e Mg das mudas. Não houve diferença, para essas características comparando-se o uso do composto de lixo urbano e o do vermicomposto. Substratos com 100% de FCV e sem adubação complementar ocasionaram sintomas de deficiência de N nas mudas de moringa.

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Most cinnamic acids, their esters, amides, aldehydes, and alcohols present several therapeutic actions through anti-inflammatory, antitumor, and inhibitory activity against a great variety of microorganisms. In this work, eight amines derived from cinnamic acid were synthesized and tested against host cells infected with Toxoplasma gondii and the bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and three strains of Staphylococcus aureus. Compounds 3 and 4 showed the best result against intracellular T. gondii, presenting antiparasitic activity at low concentrations (0.38 and 0.77 mM). The antibacterial activity of these compounds was also evaluated by the agar microdilution method, and amides 2 and 5 had a minimum inhibitory concentration of 250 µg mL−1 against two strains of S. aureus (ATCC 25923 and bovine strain LSA 88). These also showed synergistic action along with a variety of antibiotics, demonstrating that amines derived from cinnamic acid have potential as pharmacological agents.

H+ transport driven by V H+-ATPase was found in membrane fractions enriched with ER/PM and Golgi/Golgi-like membranes of Saccharomyces cerevisiae efficiently purified in sucrose density gradient from the vacuolar membranes according to the determination of the respective markers including vacuolar Ca2+-ATPase, Pmc1::HA. Purification of ER from PM by a removal of PM modified with concanavalin A reduced H+ transport activity of P H+-ATPase by more than 75% while that of V H+-ATPase remained unchanged. ER H+ ATPase exhibits higher resistance to bafilomycin (I50=38.4 nM) than Golgi and vacuole pumps (I50=0.18 nM). The ratio between a coupling efficiency of the pumps in ER, membranes heavier than ER, vacuoles and Golgi is 1.0, 2.1, 8.5 and 14 with the highest coupling in the Golgi. The comparative analysis of the initial velocities of H+ transport mediated by V H+-ATPases in the ER, Golgi and vacuole membrane vesicles, and immunoreactivity of the catalytic subunit A and regulatory subunit B further supported the conclusion that V H+-ATPase is the intrinsic enzyme of the yeast ER and Golgi and likely presented by distinct forms and/or selectively regulated.