Introduction: Postmortem personal identification is based on the extraction of deoxyribonucleic acid (DNA) from human remains or different human materials. There are some reports proving that various tissues may differ genetically and do not always have the DNA profile of the person from whom they originate due to mutation or chimerism. Aim of the work: The work aimed at the postmortem detection of the possible change in DNA fingerprint after allogeneic hematopoietic stem cell transplantation in liver tissue samples. Materials and Methods: One hundred twenty eight adult male mice weighing 25g. DNA fingerprint first was done from blood samples. After 3 months from interference, postmortem DNA fingerprinting was done again from liver tissue. Results: Fluorescent stem cells were detected postmortem in liver tissue of 30 mice by percentage of 93.75%. The change in DNA profile was detected postmortem in 20 mice in liver tissue samples by a percentage of 62.5%. Conclusion: DNA chimerism, after allogeneic hematopoietic stem cell transplantation, can be detected postmortem in the recipient's liver. Recommendations: It's recommended to study the postmortem detection of DNA chimerism in organs other than the liver to compare the percentage of chimerism detection.
In this study, a total of 209 individuals of leeches were collected from Al-Hindyia River / Babil Province. 116 individuals were identified as Erpobdella octaculata (Linnaeus, 1758), 50 individuals as Erpobdella punctata (Leidy,1870) and 43 individuals as Hemiclepsis marginata (Müller, 1774). Four samples were collected monthly during a period from February to June 2018. Some physical and chemical water properties were also examined, including air and water temperature, potential of hydrogen pH, Electrical Conductivity EC, Total Dissolved Solid TDS, Dissolved Oxygen DO, and the Biological Oxygen Demand BOD₅. Air and water temperature were ranged 19.5-29, & 14.6-23.2 °C respectively. The values of pH ranged 6.2-7.6. EC ranged 1104-1581 μs/cm². The TDS recorded 669-767 mg/l, while the DO reached 1.3-8.5 mg / l, the BOD₅ ranged 3.5-5.7 mg/l.
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