Quality seed is one of the key inputs for increasing potato (Solanum tuberosum L.) productivity; however, its limited availability is widely recognized as a major constraint to potato production. In Uganda, certified seed production meets approximately 1.5% of effective demand implying most farmers use home‐saved seed. This practice encourages the buildup of degenerative seed‐borne diseases which ultimately affect productivity. Consequently, a study was conducted to determine the productivity of different seed potato production starting stock: tissue culture plantlets (TC) and rooted apical cuttings. Fifty plants of two potato varieties, NAROPOT1 and NAROPOT4, and for each starter material were evaluated both in the field and screen house for two seasons at Kachwekano and Karengyere research stations. Data were collected on the number of tubers per plant and tuber weight. Highly significant differences were observed for the type of starter materials and varieties for the number of tubers per plant (p ≤ 0.001) from both the screen house and field environments. More tubers per plant were obtained from TC for both varieties and the highest recorded was (24.5) for NAROPOT4 and (15.5) for NAROPOT1 in the screen house. Tuber weight was higher from rooted apical cuttings for both varieties NAROPOT1 (153.3 g) and NAROPOT4 (167.7 g). The correlation between the number of tubers per plant and weight was highly significant at p ≤ 0.001. Tissue culture raised plants were more productive in terms of tuber, and numbers are more important in seed production than weight and mini‐tuber production can be done by farmers under affordable conditions.