Background: Citrus ranks top among the agricultural export commodities of Bhutan both in terms of volume and value. However, citrus cultivation practices still remain traditional with very low yield and inferior fruit quality. This study adopted community approach to identify basic components of citrus orchard management. Citrus growers of Drujegang were trained on citrus orchard management and assessed the impact of training on the adoption of management technology and subsequent effect on the yield and household (HH) income for 40 randomly selected individuals.Results: Statistical results showed significant difference both in terms of adoption of improved orchard management practices (p = 0.04) and HH income generation (p = 0.01). Adoption of improved management practices increased from 4.54 % (in 2012) to over 16 % (in 2014) with a mean yield increase of 27.5 % (212 kg acre −1 ) over previous year. Similarly, mean production increased from 5376 (2012) Conclusions:Hands-on training on basic orchard management increased the rate of adoption and resulted in increased yield and production. Huge potential exists in enhancing the livelihood of citrus growers by taking forward the available orchard management technology to growers through appropriate research and extension intervention. Therefore, replication of similar participatory approach at community level is recommended in other parts of the country.

The Himalayan uplift, a tectonic event of global importance, seemingly disseminated aquatic biodiversity broadly across Asia. But surprisingly, this hypothesis has yet to be tested. We do so herein by sequencing 1,140 base-pair of mtDNA cytochrome-b for 72 tetraploid Nepalese/Bhutanese Snowtrout (Schizothorax spp.), combining those data with 67 GENBANK sequences (59 ingroup/8 outgroup), then reconstructing phylogenetic relationships using maximum likelihood/ Bayesian analyses. Results indicate Snowtrout originated in Central Asia, dispersed across the Qinghai-Tibetan Plateau (QTP), then into Bhutan via south-flowing tributaries of the east-flowing Yarlung-Tsangpo River (YLTR). The headwaters of five large Asian rivers provided dispersal corridors into southeast Asia. South of the Himalaya, the YLTR transitions into a westward-flowing Brahmaputra River that facilitated successive colonization of Himalayan drainages: First Bhutan, then Nepal, followed by far-western drainages subsequently captured by the Indus River. We found greater species-divergences across rather than within-basins, implicating vicariant evolution as a driver. The Himalaya is a component of the third-pole [largest (but rapidly shrinking) glacial reservoir outside the Arctic/Antarctic]. Its unique aquatic biodiversity must not only be recognized (as herein) but also conserved through broad, trans-national collaborations. Our results effectively contrast phylogeography with taxonomy as a necessary first step in this process.

A field experiment on two planting methods (direct seeding and transplanting) in finger millet was conducted using two cultivars; Samtenling Memja 1 (SM1) and IE4425 at the Agriculture Research and Development Centre (ARDC), Samtenling from July to December 2020. The study aimed to determine the best planting method that gave better yield and economic advantage in crop production. The results of the experiment showed that the transplanted finger millet recorded the highest yield (0.81 Mt ha-1) compared to directed seeded (0.65 Mt ha-1) but statistically not significant. There was a percent yield difference of 19.7 % between the two methods. Similarly, there was no significant effect between planting methods and yield components such as plant height, productive tillers, and finger numbers; but there were varietal significant effects on plant height and length of the fingers (P=0.00). However, a significantly shorter maturity duration was observed in direct-seeded millet (120 days) as compared to transplanted millet (126.5 days). Moreover, economic analysis indicates that net returns for direct seeding were considerably greater (>25%) than that of the transplanting method. Based on the study, direct-seeded finger millet could be promoted in farmers' fields considering the economic advantage and early maturity.

The recurrence of similar evolutionary patterns within different habitats often reflects parallel selective pressures acting upon either standing or independently occurring genetic variation to produce a convergence of phenotypes. This interpretation (i.e. parallel divergences within adjacent streams) has been hypothesized for drainage-specific morphological ‘ecotypes’ observed in polyploid snowtrout (Cyprinidae: Schizothorax ). However, parallel patterns of differential introgression during secondary contact are a viable alternative hypothesis. Here, we used ddRADseq ( N = 35 319 de novo and N = 10 884 transcriptome-aligned SNPs), as derived from Nepali/Bhutanese samples ( N = 48 each), to test these competing hypotheses. We first employed genome-wide allelic depths to derive appropriate ploidy models, then a Bayesian approach to yield genotypes statistically consistent under the inferred expectations. Elevational ‘ecotypes’ were consistent in geometric morphometric space, but with phylogenetic relationships at the drainage level, sustaining a hypothesis of independent emergence. However, partitioned analyses of phylogeny and admixture identified subsets of loci under selection that retained genealogical concordance with morphology, suggesting instead that apparent patterns of morphological/phylogenetic discordance are driven by widespread genomic homogenization. Here, admixture occurring in secondary contact effectively ‘masks’ previous isolation. Our results underscore two salient factors: (i) morphological adaptations are retained despite hybridization and (ii) the degree of admixture varies across tributaries, presumably concomitant with underlying environmental or anthropogenic factors.

The survey was conducted in 2019 to generate baseline information for rice yield and varietal diversity, involving 54 experienced rice farmers of Singye Gewog (block) under Sarpang Dzongkhag (district) in Southern Bhutan. A total of 9 varieties were found under cultivation, of which 2 and 7 were assessed to be improved varieties and landraces respectively. The mean yield of improved varieties was 1253.2 kg acre-1, whereas landraces yielded 777.1 kg acre-1 (P=.006). The chronological varietal diversity revealed that 47.05% of farmer-named varieties were either lost or replaced over 20 years. However, landrace such as Choti Mahsino was popular, covering 24.6% of the rice cultivated area. Further, taste, yield stability, and utility for festivals were the main reasons for perpetuating landraces’ cultivation. The study showed the positive impact of improved varieties on productivity while it has narrowed varietal diversity. On the flip side, the current research found that only one improved variety was adopted which shows a need to promote improved varieties, while the improvement of landraces should also be given priority.