The development of a nanoparticle-based optical platform has been presented as a biosensor for detecting targetspecific plant virus DNA. The binding dynamics of gold nanoparticles has been studied on the amine-functionalized surface by the attenuated total reflection (ATR)-based evanescent wave absorption method monitoring the localized surface plasmon resonance (LSPR). The developed surface was established as a refractive index sensor by monitoring the LSPR absorption peak of gold nanoparticles. This nanoparticle-immobilized surface was explored to establish as a biosensing platform with target-specific immunoglobulin (IgG) antibody−antigen interaction. The IgG concentration-dependent variation of absorbance was correlated with the refractive index change. After successfully establishing this ATR configuration as an LSPR-based biosensor, the single-stranded DNA of the chilli leaf curl virus was detected using its complementary DNA sequence as a receptor. The limit of detection of this sensor was determined to be 1.0 μg/mL for this target viral DNA. This ATR absorption technique has enormous potential as an LSPR based nano-biosensor for the detection of other begomoviruses.
The development of off-stoichiometry thiol-ene-epoxy (OSTE+) based functional polymer has paramount importance for smart photonic sensors integrated lab-on-chip device applications. A modest attempt has been made to develop a semiconducting...
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