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At present, the study of the battery internal resistance at home and abroad is also a weak link, the inaccuracy of internal resistance measurement has become the biggest problem of current battery detection, which directly affects the battery capacity to play effectively, reducing the safety and reliability using of battery, making the battery charge and discharge control lack of sufficient reference. In this paper, a large number of experiment data were analyzed and sorted out, and various factors affecting the internal resistance were summed up. Conduct predictive parsing based on the summary factors and SPSS software and the multiple linear regression prediction method based on the statistical prediction was proposed to compensate resistance in access system, and take the algorithm compensation to error of the internal resistance measurement which affected by many factors. After the system applied compensation, the smallest resolution of measured resistance is 0.003mΩ. The measurement accuracy may reach to 1% under complex site conditions. The equipment meets the requirements that projects propose and realizes accurate real-time measurement of multiple battery resistance.