In this article the performance study of mustard oil bio-diesel blended with commercially available diesel has been conducted on the single cylinder engine. The bio-diesel was extracted from the mustard oil by transesterification process. The percentage yield of the biodiesel was ≈82%. The extracted bio-diesel was blended with commercially available diesel in different fractions for the study. The performance study and emission analysis were calculated on single cylinder four stroke diesel engine. According to analysis brake specific energy consumption there was decreasedand there is slight increase in brake thermal efficiency. The exhaust analysis showed that there was considerable drop in the engine emission like carbon dioxide, carbon mono oxide and hydrocarbons, resulting in lower smoke opacity. The oxides of nitrogen were present at a higher percentage in the engine emissions when the bio-diesel blend was used.

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