Three commercially available immobilized lipases, Novozym 435 from Candida antarctica, Lipozyme IM from Rhizomucor miehei, and Lipase PS-C from Pseudomonas cepacia, were used as biocatalysts for the interesterification of conjugated linoleic acid (CLA) ethyl ester and tricaprylin. The reactions were carried out in hexane, and the products were analyzed by gas-liquid chromatography. The effects of molar ratio, enzyme load, incubation time, and temperature on CLA incorporation were investigated. Novozym 435, as compared to Lipozyme IM and Lipase PC-C, showed the highest degree of CLA incorporation into tricaprylin. By hydrolysis with pancreatic lipase, it was found that Lipozyme IM and Lipase PS-C exhibited high selectivity for the sn-1,3 position of the triacylglycerol early in the interesterification, with small extents of incorporation of CLA into the sn-2 position, probably due to acyl migration, at later reaction times. A small extent of sn-1,3 selectivity during interesterification by Novozym 435 was observed.Paper no. J9718 in JAOCS 78, 547-551 (May 2001).

A confirmatory and quantitative method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of mebendazole and its hydrolyzed and reduced metabolites in pork, chicken, and horse muscles was developed and validated in this study. Anthelmintic compounds were extracted with ethyl acetate after sample mixture was made alkaline followed by liquid chromatographic separation using a reversed phase C18 column. Gradient elution was performed with a mobile phase consisting of water containing 10 mM ammonium formate and methanol. This confirmatory method was validated according to EU requirements. Evaluated validation parameters included specificity, accuracy, precision (repeatability and within-laboratory reproducibility), analytical limits (decision limit and detection limit), and applicability. Most parameters were proved to be conforming to the EU requirements. The decision limit (CCα) and detection capability (CCβ) for all analytes ranged from 15.84 to 17.96 μgkg-1. The limit of detection (LOD) and the limit of quantification (LOQ) for all analytes were 0.07 μgkg-1 and 0.2 μgkg-1, respectively. The developed method was successfully applied to monitoring samples collected from the markets in major cities and proven great potential to be used as a regulatory tool to determine mebendazole residues in animal based foods.

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(Fig. 1D) (Table 3). 조가비핵의 경우는 남녀 모두에서 왼쪽 과 오른쪽 부피차이는 보이지 않았다 (Table 4) Abstract : Corpus striatum is subcortical nuclei composed of caudate nucleus and putamen. It has been considered to be associated with motor control and learning. Dysfunction of the striatum is related to Huntington's disease, Tourette's syndrome, obsessive-compulsive disorder and schizophrenia. Nevertheless, standard Korean striatum volume was not set yet. Here, we report the striatum volume in healthy Korean youths. The subjects were composed of 57 youths (male, 28; female, 29). The MRI study was undertaken after a brief history taking and neurological examination. The DICOM files were imported into V-Works program. Volume models of the intracranial cavity, whole brain, caudate nucleus, and putamen were made and their volumes were calculated by the program.The average caudate volume was 7.23±1.18 cm 3 in male group and 6.23±0.96 cm 3 in female group. The average volume of putamen was 7.19±1.25 cm 3 in male group and 6.38±0.86 cm 3 in female group. Interestingly the right caudate volume is significantly larger in both group, although there is no difference in putamen volume. This study reports Korean corpus striatum volume in healthy volunteers. These results would provide an important standard reference for further study.