The effects of dietary supplementation of Gaeddongssuk (Artemisia annua L.) powder were evaluated in terms of performance and physicochemical properties of the chicken (Woorimatdag). The chickens were divided into five treatments: group feed basal diet (Control), group added 0.25% antibiotics in the drink water (Antibiotics), and groups supplemented Gaeddongssuk powder with 5%(TA-1), 6%(TA-2) and 7%(TA-3) in the diet. Body weight was significantly higher in TA-1 and TA-2 compared to the control(p<0.05). Feed conversion rate in the TA-1 and TA-2 groups were significantly lower compared to the control(p<0.05), but TA-3 was similar to the control. Content of crude protein was significantly higher in the TA-1 and TA-2 to the control(p<0.05). The pH of breast meat was significantly higher in the TA-2(p<0.
Agarase from a novel agar-degrading bacterium isolated from seawater in Namhae at Gyeongsangnamdo province of Korea was characterized. The SH-1 strain was selected from thousands of colonies on Marine agar 2216 media. Almost full 16S rRNA gene sequence of the agarolytic SH-1 strain showed 99% similarity with that of bacteria of Simiduia genus and named as Simiduia sp. SH-1. Agarase production was growth related, and activity was declined from stationary phase. Secreted agarase was prepared from culture media and characterized. It showed maximum activity of 698.6 units/L at pH 7.0 and 30°C in 20 mM Tris-HCl buffer. Agarase activity decreased as the temperature increased from an optimum of 30° C, with 90% and 75% activity at 40°C and 50°C, respectively. Agarase was not heat resistant. Slightly lower agarase activity was observed at pH 6.0 than at pH 7.0, without statistical difference, and 80% and 75% activity were observed at pH 5.0 and 8.0, respectively. Neoagarotetraose and neoagarobiose were the main final products of agarose, indicating that it is β-agarase. Simiduia sp. SH-1 and its β-agarase would be useful for the industrial production of neoagarotetraose and neoagarobiose, which have a whitening effect on skin, delaying starch degradation, and inhibiting bacterial growth.Key words : β-Agarase, marine bacterium, neoagarobiose, neoagarotetraose, Simiduia sp. SH-1 *Corresponding author *Tel : +82-51-999-5624, Fax : +82-51-999-5636 *E-mail : slee@silla.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.