Objective: This in vitro study was carried out to evaluate the anti-urolithiatic activity of the methanolic extract of the Cucumismelo seeds on experimentally prepared calcium oxalate crystals which was prepared by the homogeneous precipitation method in the laboratory.Methods: The crude extract was prepared by the soxhlet extraction method and the extraction was done until all the compounds get extracted into the solution and solvent was evaporated by rotary evaporator. Extracts were stored in an airtight light-resistant container at 4 °C in a refrigerator for further analysis.Results: Seed extract of Cucumismelo showed maximum efficiencies in the dissolution of the calcium oxalate crystals. Cystone drug was used as the standard. This in vitro study has shown that the methanolic extract of the seeds of Cucumismelo has the potential anti-urolithiatic activity when compared with the standard.Conclusion: This in vitro study has given the primary evidence that the extract of seeds of Cucumismelo has the anti-urolithiatic activity. In vivo studies can be carried out on the seed extract of Cucumismelo for further investigations.

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