Raman spectroscopy is an important method for identifying molecules, which is widely used in determining the chemical and structural characteristics of various substances. Many materials have a special Raman spectrum, so that this phenomenon has turned the Raman device into an efficient tool for studying the structural and chemical properties of molecules. Since it is possible to obtain detailed information about the chemical and structural characteristics of biological compounds from Raman spectroscopy, the use of this method is rapidly expanding in the field of life sciences, especially in biological and medical studies. There is no need for special, time–consuming and expensive preparations in the study of materials with the help of a Raman device. In the protein Raman spectrum, distinct bands arise from the vibrational states of the peptide backbone and amino acid side chains. Therefore, based on the position and intensity of the protein's Raman spectrum, it is possible to obtain valuable information about its second, third, and fourth structures. Also, the Raman spectrum of the protein contains information about the orientation and surrounding environment of the amino acid side chains. The correct formation of the disulfide bond in the protein structure can also be studied with the help of the Raman device. In general, the Raman spectrum of proteins contains multiple discrete bands that represent the vibrational states of the molecule and is used as a selective fingerprint to accurately determine the three–dimensional structure of proteins, intramolecular dynamics, and intermolecular interactions.

Molecular imaging is a new method in examining physiological studies in molecular dimensions. Among the various methods that have been introduced for this purpose, the magnetic resonance spectroscopy (MRS) method has made it possible to more accurately study the activities of the brain region as well as tumors in different parts of the body. MRS imaging is a type of non– invasive imaging technique that is used to study metabolic changes in the brain, stroke, seizure disorders, Alzheimer's disease, depression and also metabolic changes in other parts of the body such as muscles. In fact, since metabolic changes in the human body appear faster than anatomical and physiological changes, the use of this method can play an important role in the early detection and diagnosis of cancers, infections, metabolic changes and many other diseases. (Graphical Abstract) Graphical Abstract. CERN Large Hadron Collider (LHC) radiation source for magnetic resonance biospectroscopy in metabolic and molecular imaging and diagnosis of cancer.

The presence of electrical activity in body organs such as the nervous system, heart and bones has caused one of the most widely used treatment methods called electrotherapy to be widely used, especially in the relief of underlying pain. On the other hand, the progress of medical science in the field of nano stem cells and regenerative medicine has created many promises in treatment. Also, recently, treatments based on electric field have been widely used in cancer treatment. The main issues in regenerative medicine are the proliferation of nano stem cells to the required extent and directing them towards the differentiation of the target tissue. Electric field (EF) stimulation can play an important role in creating appropriate nano stem cell responses and directing nano stem cell differentiation towards osteogenesis/neuronogenesis/cardiomyogenesis. The electric field with nanosecond pulse as well as the electric field for tumor therapy have attracted a lot of attention for the treatment of cancer. Major signaling pathways and cellular responses elicited by electrical stimulation include reactive oxygen species and heat shock proteins, fluctuations in intracellular calcium ion concentration, ATP production, clustering or reaggregation of cell surface receptors., reconstruction of the cytoskeleton that affect the fate of the nano stem cell. Also, the lack of pain, ease and reasonable price have made cancer treatment with electric field more and more popular. In this research, an attempt has been made to briefly review the effects of electrical signals on the behavior of nano stem cells, as well as examples of their therapeutic effects in the treatment of tissue lesions and cancer. Schematic of the inverse piezoelectric effect of synchrotron light source imaging of electrical stimulation on the fate of nano stem cells and its role and application in regenerative medicine and cancer treatment.