The aim of this study was to study the biomedical activity (biofilm by fluoresence microscope) and Characterization of Selenium NPs synthesized by klebsiella pneumonia. Different types of bacterial strains (S1-S35) aureus, were screened for biosynthesis of selenium NPs. Isolate S6 was selected as efficient isolate based on color change and antibacterial activity against the pathogenic bacteria, and it was diagnosed as klebsiella pneumonia depending on the morphology, microscopic examination and VITEK2 compact system. Characterization of bio-selenium NPs was performed using: UV-visible spectroscopy was used to characterize the synthesis of selenium NPs by Klebsiella pneumonia, with an absorption peak at 280 nm. The size and structure of the bioavailable selenium NPs were measured using an XRD occurring at 20°-26°. AFM analysis showed that the mean diameter of the bio-selenium NPs was 47. 18. Antibacterial activities have been studied for bio-selenium against pathogenic bacteria (B. cepacia, S. aureus, A. baumanni and E. coli).Biogenic Selenium expressed high significant antibiofilm activity with increasing concentration of Selenium NPs. DPPH reducing activity of nanoparticles which increased with increase concentration of biogenic Selenium NPs.

The purpose of this research was to study the biological activity of probiotic against MDR bacteria that isolated from diabetic foot infections. During the months of October 2020 to March 2021, a total of 65 specimens and investigations were collected. Specimens for diabetic foot infection were gathered from two hospitals in the province of AL-Najaf (AL-Sadder Medical City and AL-Hakeem Hospital). Swabs from diabetic foot infection patients were collected and sent to the lab, where they were streaked on MacConkey agar, a blood agar., around 40 (61.5%) exhibit positive bacterial growth culture, whereas 25 (38.5%) show negative findings for culturing. Negative growth might be due to other infection-causing agents such as anaerobic bacteria, fungi, and viruses  [1].  Gram-negative bacteria had a high rate of 42 (64.4%) based on morphological and biochemical tests, with B. cepacia having a high percentage of 14 (33.3%) and A. baumanni having a high percentage of 12 (28.7%). K. pneumonia has 7 (16.6%), E. cloacae has 6 (14.4%), and no growth in 3 (7%) as shown in figure (4-2).