The cultivation of the olive tree has several problems related to pests and diseases that can cause heavy economic losses by causing the death of trees. Fusarium solani was detected in the olive trees of Sidi Taibi's nurseries located in the national road between Kénitra and Rabat in spring 2012 and 2013 (1 to 2% of diseased plants). Koch's postulate was verified in the olive trees variety 'Picholine Moraine', inoculated with F. solani. Three months after inoculation of the plants, the extremities of the young buds began to dry out, then the drying became generalized and the roots of the inoculated plants showed rot and detached from the base of the plants. The pathogen was re-isolated from the roots, dried buds, bark and petioles of the leaves of the inoculated plants; the percentage of isolation was 100%.
Fungal isolates of Fusarium were collected from symptomatic chickpea (Cicer arietinum L.) plants growing in fields within Souk Tlat commune in the Gharb region. Morphological and molecular characterizations were performed of the fungal isolate N3 obtained from a chickpea plant. PCR amplification and sequencing of the internal transcribed spacer using the primers ITS1 and ITS4 was applied to identify the fungal isolate N3. The maximum similarity index of the fungus was found to be 99.33% with Fusarium equiseti (accession no. MT111122). In the pathogenicity test, both chickpea seed dip inoculation and soil infestation by the spore suspension of Fusarium isolate were adopted. Four weeks after chickpea seed inoculation, few plants emerged and those that emerged were stunted. A high percentage of inoculated seeds did not emerge and showed accentuated rot symptoms. Eight weeks after sowing seeds in infested soil, the obtained chickpea seedlings displayed root necrosis, browning at the crown, and wilting. In addition, these plants showed a foliar alteration index of 0.395. The re-isolation was positive for different parts of chickpea plants for both seed and soil inoculation. Fusarium equiseti isolate decreased the length of the root and aerial parts, and number of leaves and branches of the inoculated chickpea plants either by seed inoculation or soil infestation with values of 0.91 cm and 19.73 cm, 1.29 cm and 19.44 cm, 1.11 and 18.66, and 0.0 and 2.08 respectively, whereas the corresponding values for the control plants were 27.16 and 28.33 cm, 29.05 and 31.05 cm, 24.21 and 25.66, and 3.50 and 3.11, respectively. To the best of our knowledge, this is the first report of F. equiseti on chickpea (Cicer arietinum L.) in Morocco.
In the greenhouse, mixed inoculation of Tetraclinis articulata (Vahl) Mast.) with Rhizoctonia solani Kühn and Fusarium solani (Mart.) Sacc. caused remarkable damping-off and root rot compared to simple inoculation with one of the two pathogens. Root and stem infections caused significant reduction in plant growth. Root system total length was reduced by 36 to 43% and shoot height losses by 28 to 39%. Plants inoculated by one pathogen were shorter than control plants or inoculated plants by both pathogens. Disease severity ratings for plants infested with the pathogens mixture also were greater than with either pathogen alone up to 62 days post-inoculation. R. solani and F. solani interact synergistically, causing severe damping-off and root rot in T. articulata seedlings.
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