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ABSTRACT. The filamentous fungus Moniliophthora perniciosa is a basidiomycota that causes the witches' broom disease in cocoa trees (Theobroma cacao L.). The mitochondrial DNA polymerase of M. perniciosa (MpmitDNApol) is classified within the B family of DNA polymerases, which can be found in viruses and cellular organelles. Using virtual screening processes, accessing KEGG, PubChem, and ZINC databases, we selected the 27 best putative nucleoside viral-like polymerase inhibitors to test against MpmitDNApol. We used Autodock Vina to perform docking simulations of the selected Then, we used Pymol v1.7.4.4 to check the stereochemistry of chiral carbons, hydrogen bonding receptors, absence or presence of hydrogen, sub and superstructure, numbers of rings, rotatable bonds, and donor groups. We selected the Entecavir Hydrate, a drug used to control hepatitis B; subsequently AMBER 14 was used to describe the behavior of polymerase-entecavir complex after setting up 3500 ps of simulation in water at a temperature of 300 K. From the simulation, a graph of Potential Energy was generated revealing that the ligand remains in the catalytic site after 3500 ps with a final energy of -612,587.4214 kcal/mol.

Introduction Many authors have written about the need to treat patients closer to their beds, in order to observe them more as distinct people. The FAST HUG mnemonic, which consists of a checklist, was suggested as an idea to be employed everyday, by professionals dealing with patients who are critically ill. Minding these questions and motivated by an idea of follow patients' treatment closer, we have put into practice the instrument developed by Jean-Louis Vincent, evaluating the seven most important procedures in critically ill patients, and performed the FAST HUG. This checklist consists of seven items to be evaluated: Feeding, Analgesia, Sedation, Thromboembolic prophylaxis, Head-of-bed elevation, stress Ulcer prevention, and Glucose control. Knowing that the pressure ulcer is one of the challenges faced by ICU nurses, related to patients' need to stay at rest, to be under rigorous control or more complex therapy, it was decided to create the eighth item on the checklist: S, for skin. It stands for skin treatment, with the techniques used in the unit (Braden Scale), monitoring and evaluating closer skin integrity, and allowing nurses to calculate the scoring average of the Braden Scale, and greater incidence of ulcer in interned patients. Objective To expose the shortcomings found during the FAST HUG application, and to show results obtained with the eighth item of the FAST HUG mnemonic: S-Skin. Methods A descriptive study, based on institutional data, was carried out in the adult ICU of a private hospital. It was performed from 2 to 27 June 2008, except on weekends. Three hundred and twenty-three patients were involved. The checklist was carried out during the afternoons by the head nurse, or the assistant nurse of the unit. In order to do this job, a spreadsheet was elaborated to control data, updated every week. This spreadsheet provided graphics for a more objective control of the results obtained. The idea was exposed to the team, during a training program, and so we started the activities. Results and discussion For 20 days of the checklist, 323 patients were evaluated for the eight items. The real shortcomings most frequently found were related to thromboembolic prophylaxis (85%) and glucose control (90%). These shortcomings were immediately evaluated and, depending on this analysis, this item would go on or not, according to the patient's clinical situation. The shortcomings found were tracked just as they were detected, and their cause would be discussed in a multidisciplinary group, and a solution was found. If the item was not observed, it would be 1. Vincent JL: Give your patient a fast hug (at least) once a day.

IntroductionSyphilis persists as a major and ascendant health issue. However, the impact of this disease during the adolescence, a period of behavioural, sexual and psychologic vulnerabilities, is still underexplored. We aimed to evaluate the follow-up of syphilis infected adolescents attended at a reference service of sexually transmitted infections (STI).MethodsA retrospective cohort study using data from medical records of adolescents (10 to 19 years old) with diagnosis of syphilis attended at the states STI reference centre, from January to August 2012.ResultsAmong 776 adolescents attending the service, 58 had a diagnosis of syphilis (7.47%). The majority was male (51.7%), mean age was 16.8 (±1.35) years, 92.3% attended school, and 58.1% had ≤8 years of schooling; 39.4% referred drinking alcohol and 39.5% drug use. Among the adolescents, 98.3% had already initiated sexual life, with the mean age of sexual debut of 14.2 (±1.48) years, with an average of 2.33 (±2.55) lifetime sexual partners. About 14.0% declared to be homosexual, 85.4% referred irregular condom use, 22.2% of the girls were pregnant, with mean age at first gestation of 16.0 (±1.09) years. A parcel of 4.8% of the adolescents were HIV-infected, 31.0% reported a previous STI (p=0.02, OR 2.68, 95% CI 1.16–6.17), and 52.3% had another current STI. The mean number of medical visits was 2.33, 22.8% had primary syphilis, 17.5% had the secondary phase of the disease and 59.6% had latent or late syphilis. The social and demographic variables were not statistically different among the adolescents in the different stages of syphilis. The genital ulcer complaint was related to the diagnosis of primary or secondary syphilis (p=0.01, OR 8.53, 95% CI 1.61–45.1). 91.4% of adolescents received treatment for syphilis and 56.6% performed a cure control.ConclusionThe high prevalence of other STIs associated with syphilis in adolescents demonstrates the limited knowledge of this population to care and prevention strategies, and remains a challenge for specialised services in the diagnosis and treatment of STI/HIV.