The H . integrifolia leaves yielded hexacosanol, octacosanol, B-sitosterof and ,6-amyrin while the stem bark yielded friedelin and friedelan-3P-01.Holoptelea integrifolia PLANCH. syn. Ufmus integrifolia ROXB. (Ulmaceae), commonly known as Chilbil, is the only species of the genus found in India. It is an avenue tree distributed (BRANDIS, 1971) throughout the country up to an altitude of 2000ft. The tree produces medium quality timber, the pulped bark is used for making hardboard (Wealth of India, 1957) and the leaves are eaten by cattle. The bark is reported to yield fi-sitosterol (GUPTA et al., 1969) only. The seeds contain 37.4% of a fixed oil (Wealth of India, 1957) and the seed cake is rich in protein, 64.3% consisting of 3.3% lysine (GOVINDRAJAN and RAMACHANDRAN, 1952), 13% glutamic acid and 1.3% histidine (KRISHNAMACHAR and RAMACHANDRAN, 1954). The analyses of the leaves as fodder are also recorded. The pollen grains of the flowers of the tree are reported (SHIVAPURI, 1964) allergen.A very interesting 0-glucoside-C-glucoflavone, tremasperin was for the first time reported (OELRICHS, 1968) by a Cambridge group of workers from the leaves of Trema aspera (Ulmaceae) which encouraged us to take up the investigation of leaves and bark of another ulmaceous plant, Holoptelea integrifolia.
Constituents of leavesThe coarsely powdered shade dried leaves (5.5 kg) were sequentially percolated with hexane and alcohol. he alcoholic concentrate was segregated into hexane and ether soluble fractions.The hexane soluble fraction was combined with the hexane extract and processed to yield a dark coloured thick liquid (80 g) which on chromatography over alumina gave a waxy product (11.8 g), m. p. 55-67'.The waxy product on rechromatography yielded four compounds. The compound. (4.2 g) m. p. 75-77', on purification through crystallizations finally melted at 76-78' and was identified as hexacosanol by superposable IR 3408 (OH) and 721 (alkane chain) cm-I, co-TLC (NIGAM and MITRA, 1968) and acetate, m. p. 62-63'.
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