Ascorbyl-6-palmitate (AP) was a lipid-soluble synthetic ester of ascorbic acid that has been used as a preservation agent for foods and as an antioxidant in cosmetics and related products. This study aims to investigate the effect of shearing management and ascorbyl palmitate administration on physiological and hematological profiles in under heat load Padjadjaran sheep. The physiological profile involves respiration rate (RR), Pulse rate (PR), and rectal temperature (RT). Twenty rams Padjadjaran 1 – 1,5 year-old, weighing between 25 to 47.5 kg used from sheep breeding station Purwakarta, West Java - Indonesia. There are two treatments level in this research, (1). Shearing divide into two groups, unshearing (c0) and shearing (c1); (2). AP administration also divides two groups, not given (a0) and given 400 mg each head daily (a1). The whole treatments were a0c0, a0c1, a1c0 and a1c1. Heat exposure application was exposed to sunlight when the sheep exposed, the animal tethered by rope at the neck region. The sheep exposed to the sun when the ambient temperature at least 34oC, 180 minutes each day, three days a week. The result showed that AP administration and shearing have interaction effects on RR, PR and RT. The level of glucose and total protein is affected by interaction effect of AP administration and shearing, while cholesterol affected to shearing. The conclusion, supplementing AP and shearing application could reduce the negative impact of heat stress on physiological and blood metabolite profiles of Padjadjaran sheep. Although the wool of local breed has not economic value, the shearing is regularly needed.

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The diversity of leptin genes in cattle has an impact to the physiological function of the leptin hormone as anti-obesity. This study was aimed to determine polymorphisms of the Leptin gene based on Single Nucleotide Polymorphism on exon 3rd and their effect to body weight, of Padjadjaran sheep. DNA samples were obtained from the blood of 26 Padjadjaran rams 1 years. Data of leptin gene diversity were analyzed from the results of PCR product sequencing by Sanger Methode of 696 bp leptin exon 3. Point mutations (SNPs) on nucleotide sequence were analyzed using Bioedit and MEGA 7 Program. Growth parameters which were measured i.e. monthly body weight gain until one year of age. The association of leptin gene polymorphism with body weight was find out by unpaired T test. The results showed there were SNPs in Leptin gene in exon 3 of Padjadjaran sheep, namely g.170 G> A and g. 332.A> G. Both of these mutations are non-synonymous mutations because there were altered amino acids from Arginine to Glutamine. Genotyping was done based on the two mutations above and got two alleles G and A, each of which has an allele frequency of 0.75 and 0.25, with the frequency of each genotype, AG and GG was 0.5. There was not association between SNPs on leptin gene with body weight from birth to 12 months of age.