<p><em>Pleurotus ostreatus</em> is an edible mushroom that also has potential as medicinal values. In this study, fruiting body of <em>P. ostreatus</em> was tested for its ability to inhibit the growth of fungi and bacteria. The fruiting body powder of <em>P. ostreatus </em>was extracted using methanol by maceration method. Analysis of this compound was done by using anisaldehid sulfuric acid, Dragendorff reagent, and FeCl₃. Using the agar well diffusion technique, the extracts were tested for their antimicrobial activity against <em>Staphylococcus aureus </em>(Gram positive), <em>Enterobacter aerogenes</em> (Gram negative) and <em>Candida albican</em> (yeast). The spot results on TLC using crude extract of <em>P. ostreatus</em> is terpenoids. Zone of inhibition for the various extracts varied between 10.9 - 23.2 mm. Ten miligrams extract exhibit maximum antimicrobial activity against most of the tested pathogens </p>