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Nurhatika S, Ermavitalini D, Saputro TB, Apriyatmoko Y. 2018. Biodiversity and characterization of high lipid content microalgae in Porong River Estuary East Java, Indonesia. Biodiversitas 19: 577-582. Microalgae are aquatic unicellular or multicellular microscopic photoautotroph organisms. High lipid content in microalgae biomass can be used as raw material for biodiesel. Porong river estuary is one of the sites of industrial waste disposal. The polluted waters considered will affect the lipid microalgae metabolism. The main research aims are to isolate and characterize of high lipid content microalgae found in Porong river estuary through the selection of qualitative lipid content. Sampling was conducted at three different stations. Isolation was conducted in solid agar media enriched with Porong River Estuary sterile water and using streak plate method. The isolated microalgae were characterized using a light microscope and to be matched with identification books. The lipid content was determined qualitatively by coloring the isolated-microalgae cells with Nile Red, an intracellular lipid dye then observed its luminescence color under a fluorescence microscope. Several genera of microalgae that can be isolated in this study are Oscillatoria, Nitzschia, Merismopedia, Navicula, Nannochloropsis, and Melosira. The results of qualitative lipid analysis show that the genus Nannochloropsis and Nitzschia have high intracellular lipid accumulation.

Salah satu upaya pemanfaatan lahan pasir di Indonesia adalah budidaya cabai, namun rendahnya unsur hara dalam pasir merupakan kendala utama. Aplikasi mikoriza arbuskula adalah salah satu solusi alternatif untuk meningkatkan produktivitas tanaman di tanah pasir. Tujuan penelitian ini untuk mengetahui pertumbuhan cabai rawit (Capsicum frutescens) yang ditanam di tanah pasir dengan penambahan pupuk mikoriza berbeda dosis yaitu 0g, 2g, 4g, 6g dan 10g serta kontrol positif menggunakan tanah taman. Adanya pengaruh dosis mikoriza terlihat dari parameter pengamatan meliputi tinggi, luas daun dan berat kering tanaman. Persentase akar terinfeksi dan jumlah spora juga diamati untuk mengetahui proses infeksi mikoriza pada tanaman cabai rawit. Hasil penelitian menunjukkan bahwa budidaya cabai rawit di tanah pasir dengan penambahan 6g mikoriza sudah mampu meningkatkan hasil pertumbuhan namun belum bisa disamakan dengan pertumbuhan C. frutescens yang ditanam di tanah taman. Infeksi akar dan jumlah spora paling banyak terdapat pada cabai rawit dengan pemberian 10g mikoriza. Kata Kunci-Capsicum frutescens, mikoriza, tanah pasir.

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