To realize effective governance, the central government has issued a policy to simplify the bureaucracy. One of these policies is implemented by equalizing Administrative Positions (JA) to Functional Positions (JF). The Provincial Government of South Kalimantan has followed the mechanism for equalizing JA to JF following the provisions of the applicable laws. However, in its implementation, it is still partially done. The equalization of JA to JF has been carried out by four agencies, namely Bappeda, Balitbangda, Dispersip, and DPMPTSP, with the number of JAs being equalized to as many as 32 people. The problem faced by the South Kalimantan Provincial Government after the implementation of equalization is that the work system needs to be adjusted. In addition, implementing a policy that is still partial creates jealousy, inequality and work demotivation. This also impacts the irrelevance of the job made and approved by the Ministry of Home Affairs to be implemented at the next inauguration due to mutations, promotions, and retiring employees. Related to this, the provincial government must immediately make (1) adjustment to the work system, which includes adjustment of work mechanisms and business processes as stated in PermenPAN-RB RI Number 7 of 2022 concerning Work Systems in Government Agencies for Bureaucracy Simplification, (2) Preparation of guidance maps /mapping of JF training and development priorities in each regional apparatus, especially for JF who must have certification at the JF that is being supervised, (3) Conducting consultations and rearranging the JA map that will be aligned by providing opportunities for JAs who will be equalized to then choose JFs that are in demand and adapted to the needs of the organization, (4) related SKPDs to conduct intensive and pro-active guidance in conducting training on equalization JFs in their respective SKPDs and accelerating the internalization of changes in ASN management policies. Pemerintah pusat dalam rangka mewujudkan tata Kelola pemerintahan yang efektif, telah mengeluarkan kebijakan penyederhanaan birokrasi. Salah satu kebijakan tersebut dilaksanakan melalui penyetaraan Jabatan Administrasi (JA) ke Jabatan Fungsioal (JF). Pemerintah Provinsi Kalsel telah mengikuti mekanisme penyetaraan JA ke JF sesuai dengan ketentuan perundangan yang berlaku, namun dalam pelaksanaannya masih dilakukan secara parsial. Penyetaraan JA ke JF telah dilaksanakan terhadap 4 SKPD, yaitu Bappeda, Balitbangda, Dispersip, dan DPMPTSP, dengan jumlah JA yang disetarakan adalah sebanyak 32 orang. Permasalahan yang dihadapi Pemprov Kalsel setelah pelaksanaan penyetaraan adalah belum adanya penyesuaian sistem kerja. Selain itu Pelaksanaan kebijakan yang masih parsial juga menimbulkan kecemburuan, kesenjangan dan demotivasi kerja bagi JF penyetaraan. Hal tersebut ini juga berdampak pada tidak relevannya peta jabatan yang telah dibuat dan disetujui oleh Kementerian Dalam Negeri untuk dilaksanakan pada pelantikan selanjutnya karena adanya mutasi, promosi, dan pegawai yang pensiun. Terkait hal tersebut, pemprov harus segera melakukan : (1) penyesuaian sistem kerja yang meliputi penyesuaian mekanisme kerja dan proses bisnis seperti tertuang dalam PermenPAN-RB RI Nomor 7 Tahun 2022 tentang Sistem Kerja pada Instansi Pemerintah untuk Penyederhanaan Birokrasi,(2) Penyusunan peta pembinaan /pemetaan prioritas pelatihan dan pengembangan JF pada masing-masing perangkat daerah, terutama bagi JF yang harus memiliki sertifikasi di JF yang diampu, (3) Melakukan konsultasi dan penyusunan ulang peta JA yang akan disetarakan dengan memberikan kesempatan bagi JA yang akan disetarakan untuk selanjutnya memilih JF yang diminati dan disesuaikan dengan kebutuhan organisasi, (4) SKPD terkait agar melakukan pembinaan secara intensif dan pro aktif melakukan pembinaan terhadap JF penyetaran di SKPD nya masing-masing dan mempercepat internalisasi perubahan kebijakan manajemen ASN.

In order to compare transient expression of gus gene driven by CaMV 35S and rice ubiquitin RUBQ2 promoters, a DNA transformation was conducted using embryogenic callus and suspension cultures of sugarcane. The transient gus expression was observed by histochemical staining method. The histochemical observation of GUS activity after co-cultivation showed that RUBQ2 promoter produced high level of clear blue spots both in embryogenic callus and suspension cultures, while the CaMV35S promoter was not detected. The suspension cultures slightly increased transient gus gene expression compared to embryogenic callus. However, the histochemical analysis of regenerated putative transformant plants after 5 successive cycles on the selection medium showed no blue spots of gus gene expression. PCR amplification of DNA for CaMV35 or nptII in putative transformant plants confirmed that there was no integration of the transformed gene in the genome DNA. The results suggested a possibility of somaclonal variation with callus propagation, thus did not produce transformed plants. To avoid the somaclonal variation, the transformation was conducted using in vitro plants and multiple shoots without intervening callus phase. Histochemical observation of infected materials after co-cultivation showed that almost all of the infected materials partially exhibited blue color in the basal region. In case of in vitro plants, they rapidly grow and multiplied in the selection medium, thus the method provided an excellent system for the transformation in sugarcane. The results suggest that in vitro plants as well as multiple shoots need further investigation to be used as target tissues for Agrobacteriummediated transformation in sugarcane.

Agricultural land has an important role in life, especially in providing food for the community, therefore government policies, both central and regional are needed in maintaining its existence. The policy of protecting sustainable agricultural food land was one of the efforts, therefore it was necessary to carry out sustainable management of the land. This study aimed to obtain a policy needs structure model on sustainable food agriculture land in Banjar Regency, South Kalimantan. Requirement structure design was needed in the framework of sustainable management policies. Requirement structure modeling was done using Interpretative Structural Modeling. It was a strategic modeling technique that can map system conditions comprehensively. The results of the analysis showed that there was  an element of need that can make the policy successful. There were also,  the sub-element of supervision, control, coaching, development and research activities. Based on the driven power, the sub-elements that are the key factors in the elements of the need for sustainable food agriculture land in Banjar Regency were coaching and supervision.

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