Honey is consisting of simple sugar, such as glucose, sucrose, and fructose, that could serve as a source of nutrition for sperm cells during preservation. The purpose of the study was to assess the effect of honey supplementation in the extender on the spermatozoa abnormality of brek fish Systomus orphoides (Valenciennes, 1842) 48 h after preserved at 4 °C. The brek fish semen was obtained using the hand stripping method, and the diluent used is a mixture of fish ringers, 10% Methanol, and different concentrations of Honey solution (5%; 10%; 15%; and 20%). Furthermore, equilibration was carried out at 28 ºC for 1 minutes, and the sperm storage was carried out at 4 ºC for 48 h. The evaluation of semen was carried out macroscopically (color, volume, and pH) and microscopically (abnormalities). The results of the ANOVA test followed by the Tukey test was significantly different (p <0.05) in the abnormality. The results showed that treatment of 5% honey solution was the optimum concentration because the lowest abnormality was (11.85±0.45%).

Javaen barb fish Systomus orphoides Valenciennes, 1842 (Cypriniformes: Cyprinidae) is a freshwater fish whose population is declining and threatened with extinction. In this study, the ultrastructure of spermatozoa of Javaen barb fish (S. orphoides) was studied using transmission and scanning electron microscopy. The spermatozoa of S. orphoides are relatively simple cells composed of a spherical head, a short midpiece, and a flagellum, as in most Cyprinidae. The ultrastructure is characterized by the absence of acrosome, the total length of spermatozoa is 27.16 ± 4.5 μm, and the head has spherical with a length of 1.84 ± 0.10 μm and width of 1.55 ± 0.15 μm containing a nucleus, midpiece region containing the proximal and distal centrioles and mitochondria. Two or three mitochondria surrounding the axoneme (with a 9 + 2 microtubular pattern). Ultrastructural analyses by SEM and TEM of Javaen barb fish spermatozoa cells are very consistent with those of Cyprinidae. This study provides the ultrastructure information of S. orphoides spermatozoa in the Cyprinidae family this research could be useful in increasing reproductive efficiency and further prevent the extinction of this species.