Spine gourd is a perennial, dioceous climbing creeper belongs to family Cucurbitaceae and commonly known as Kartoli. The present study aimed to investigate the effect of different concentrations of IBA, types of cutting and rooting media on days required for sprouting, survival percentage growth of cuttings of kartoli. The cuttings taken from the tip, middle and basal portion of the vine were dipped into 250, 500, 750 and 1000 ppm of IBA (Indole-3-butyric acid) solution and planted in different rooting media such as Soil + Sand + FYM, Soil + Sand + Vermicompost and Soil + Sand + Cocopeat in 1:1:1 proportion. The experiment was laid out in Factorial Completely Randomized Block Design with two replications. The observations on days required for sprouting, survival percentage, length and number shoots and roots per cutting were recorded at 30 and 90 DAP. The data revealed that, the days required for sprouting, survival percentage and growth of cuttings of kartoli were found to be significantly influenced by the different concentrations of IBA, types of cutting and rooting media. Interactions between IBA concentrations (G), types of cutting (C) and rooting media (M), the treatment combination of G 4 C 1 M 1 recorded significantly minimum number of days (10.15) required for sprouting, the highest survival percentage (80.00) as compared with the remaining treatment combinations. The maximum length of main shoot and root were recorded in the treatment combination of G 4 C 1 M 1 (153.15 cm and 27.80cm), respectively and the highest numbers of shoots (6.10) and roots (132.80) per cutting were noticed in the same treatment combination as compared with the other treatments under study at 90 DAP.

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Tagging of pod shattering/dehiscence tolerance trait in soybean (qPDH loci) with molecular markers was undertaken in a segregating population of cross cultivar Kalitur (shattering of 12.02% at field level; 77.71% on oven drying) x DS-9712 (shattering of 1.63% at field level; 10.94% on oven drying) by bulked segregant analysis (BSA). Pod shattering was partially dominant over the tolerance in the cross. Inhibitory epistasis of two major genes was evidenced from F2 ratio (13:3) (3.0-16.0% shattering field level and 5-85% on oven drying), with chi-square test indicating goodness of fit which was confirmed by test cross. Only two of the five shortlisted microsatellite markers from previous study viz., Satt674 and SRM1generated polymorphism between shattering tolerant and susceptible parents and their corresponding F2 bulks. Satt674 marker alleles were closely placed (223 bp vrs. 228 bp) hampering clear resolution and hence this primer was not used forfurther validation. SRM1 primer yielded distinct polymorphic markers (237bp vrs. 225 bp) between shattering-tolerant and susceptible parents and bulks. SRM1 marker was validated in 60 individual F2 plants which were contrasting for pod dehiscence trait. Forty-seven susceptible plants yielded either only 237 bp bands or were heterozygous (having both 237 bp and 225 bp bands), while 13 shattering tolerant F2 plants amplified only 225 bp marker. Hence, in future this SRM1-225bp marker linked to a major pod shattering tolerance loci qPDH1, could be utilized for screening the parental lines, segregating generations, breeding lines and varieties of soybean.

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