Background: Hepatitis B virus infection is a global health problem and it is estimated that one-third of the world population has been infected with HBV with serological evidence of past or present infection. It is estimated that over 400 million people are chronically infected with Hepatitis B globally. Currently, its prevalence among the Ugandans is about 10% (3.5 million people). The aim of this study was to determine the prevalence and Health workers' perspective on Hepatitis B in Ngora District, Eastern Uganda.