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Frontalis flap advancement is a technically simple, safe, and effective technique for the repair of myogenic ptosis. The primary advantage of frontalis muscle flap advancement over a graft or suture material that it elevates the eyelid directly by moving the insertion of the frontalis muscle into the eyelid, rather than by graft or suture material.

Flow cytometric analysis of DNA content was performed on nuclear suspensions prepared from fresh and from paraffin-embedded, formalin-fixed lymphoid tissues. We confirmed previous reports that it is possible to obtain nuclear suspensions from deparaffinized, formalin-fixed tissues, suitable for DNA analysis by flow cytometry. We observed a tendency for a larger coefficient of variation (CV) of the DNA measurements in the fixed tissues than in the unfixed material causing abnormalities in 2 of 19 lymphomas to become undetectable. Furthermore, samples from different paraffin blocks of a single tumor with an extra G1 (hyperdiploid) peak showed marked differences in the CV of the hyperdiploid peak while the CV of the diploid peak was similar in all samples. In both benign and malignant lymphoid tissues, the Sphase fraction was higher in paraffin-embedded tissues than in unfixed cells. This differ--Cellular DNA content in human lymphoma cells has been analyzed extensively using flow cytometry (FCM). These studies have been performed primarily on cell suspensions obtained from unfixed tissues (3,4,(7)(8)(9)(16)(17)(18) and have provided information on ploidy changes and cell kinetic properties which are valuable in the detection and classification of lymphoid tumors. Recently, flow cytometric analyses of DNA content in cells from formalin-fixed, paraffin-embedded tissues have been successfully performed (2,6,10-12,151. This technique has allowed the retrospective clinical study of patients with a variety of neoplasms, including lymphomas (2,15). In our study, we applied this methodology to both neoplastic and nonneoplastic human lymphoid tissues. We tried to reproduce the analytical conditions used in the first flow cytometric study of DNA content in paraffin-embedded tissue by Hedley et al. (121, who used a mercury arc-based system and DAPI staining. The results were compared to those from unfixed samples from the same tissues. Since all unfixed lymphoma tissues had been previously analyzed on a laser-based ence could be attributed to 4', 6'-diamidino-2 phenylindole dihydrochloride (DAPI), a DNAbinding dye commonly used in this technique. Nevertheless, intermediate and high grade lymphomas from paraffin-embedded tissues generally showed a greater S-fraction than low grade lymphomas, a similar observation as with unfixed tissues. Therefore, DNA content analysis of nuclei extracted from paraffin sections may be inadequate to resolve slight aneuploidy, but the measurement of Sfraction size may remain diagnostically or prognostically valuable. Large retrospective studies will be necessary to determine the clinical impact of this technique in the analysis of lymphomas.

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