A critical challenge to the cold dark matter (CDM) paradigm is that there are fewer satellites observed around the Milky Way than found in simulations of dark matter substructure. We show that there is a match between the observed satellite counts corrected by the detection efficiency of the Sloan Digital Sky Survey (for luminosities L 340 L) and the number of luminous satellites predicted by CDM, assuming an empirical relation between stellar mass and halo mass. The "missing satellites problem", cast in terms of number counts, is thus solved. We also show that warm dark matter models with a thermal relic mass smaller than 4 keV are in tension with satellite counts, putting pressure on the sterile neutrino interpretation of recent X-ray observations. Importantly, the total number of Milky Way satellites depends sensitively on the spatial distribution of satellites, possibly leading to a "too many satellites" problem. Measurements of completely dark halos below 10 8 M , achievable with substructure lensing and stellar stream perturbations, are the next frontier for tests of CDM.

Merging galaxy clusters have been touted as one of the best probes for constraining selfinteracting dark matter, but few simulations exist to back up this claim. We simulate equal mass mergers of 10 15 M halos, like the El Gordo and Sausage clusters, with cosmologicallymotivated halo and merger parameters, and with velocity-independent dark-matter selfinteractions. Although the standard lore for merging clusters is that self-interactions lead to large separations between the galaxy and dark-matter distributions, we find that maximal galaxy-dark matter offsets of 20 kpc form for a self-interaction cross section of σ SI /m χ = 1 cm 2 /g. This is an order of magnitude smaller than those measured in observed equal mass and near equal mass mergers, and is likely to be even smaller for lower-mass systems. While competitive cross-section constraints are thus unlikely to emerge from offsets, we find other signatures of self-interactions which are more promising. Intriguingly, we find that after dark matter halos coalesce, the collisionless galaxies (and especially the Brightest Cluster Galaxy [BGC]) oscillate around the center of the merger remnant on stable orbits of 100 kpc for σ SI /m χ = 1 cm 2 /g for at least several Gyr, well after the clusters have relaxed. If BCG miscentering in relaxed clusters remains a robust prediction of SIDM under the addition of gas physics, substructure, merger mass ratios (e.g., 10:1 like the Bullet Cluster), and complex cosmological merger histories, the observed BCG offsets may constrain σ SI /m χ 0.1 cm 2 /g-the tightest constraint yet.

Despite widespread interest in using human induced pluripotent stem cells (hiPSCs) in neurological disease modeling, a suitable model system to study human neuronal connectivity is lacking. Here, we report a comprehensive and efficient differentiation paradigm for hiPSCs that generate multiple CA3 pyramidal neuron subtypes as detected by single-cell RNA sequencing (RNA-seq). This differentiation paradigm exhibits characteristics of neuronal network maturation, and rabies virus tracing revealed synaptic connections between stem cell-derived dentate gyrus (DG) and CA3 neurons in vitro recapitulating the neuronal connectivity within the hippocampus. Because hippocampal dysfunction has been implicated in schizophrenia, we applied DG and CA3 differentiation paradigms to schizophrenia-patient-derived hiPSCs. We detected reduced activity in DG-CA3 co-culture and deficits in spontaneous and evoked activity in CA3 neurons from schizophrenia-patient-derived hiPSCs. Our approach offers critical insights into the network activity aspects of schizophrenia and may serve as a promising tool for modeling diseases with hippocampal vulnerability. VIDEO ABSTRACT.

Sponges are the most conspicuous component of the Antarctic benthic ecosystem, a system under stress both from climate change and fishing activities. Observations over four decades are compiled and reveal extremely episodic sponge recruitmentand growth.Recruitment occurred under different oceanographic conditions on both sides of McMurdo Sound. Most of the sponges appear to have recruited in the late 1990s-2000. Observations from 2000 to 2010follow thirty years of relative stasis with very little sponge recruitment or growth followed by a general pattern of recruitment by some forty species of sponges. That there was almost no recruitment observed on natural substrata emphasizes the contrast between potential and realized recruitment. This unique data set was derived from a region noted for physical stasis,but theepisodic ecological phenomena highlightthe importance of rare events. Against a background of intermittent food resources and the low metabolic costs of stasis, understanding the causes of irregular larval supply, dispersal processes, recruitment success and survivorship becomes critical to predicting ecosystem dynamics and resilience in response to increasing environmental change. Our time-series emphasizes that long-term data collection is essential for meaningful forecasts about environmental change in the unique benthic ecosystems of the Antarctic shelf.5

Fast ice plays important physical and ecological roles: as a barrier to wind, waves and radiation, as both barrier and safe resting place for air-breathing animals, and as substrate for microbial communities. While sea ice has been monitored for decades using satellite imagery, high-resolution imagery sufficient to distinguish fast ice from mobile pack ice extends only back to c. 2000. Fast ice trends may differ from previously identified changes in regional sea ice distributions. To investigate effects of climate and human activities on fast ice dynamics in McMurdo Sound, Ross Sea, the sea and fast ice seasonal events (1978–2015), ice thicknesses and temperatures (1986–2014), wind velocities (1973–2015) and dates that an icebreaker annually opens a channel to McMurdo Station (1956–2015) are reported. A significant relationship exists between sea ice concentration and fast ice extent in the Sound. While fast/sea ice retreat dates have not changed, fast/sea ice reaches a minimum later and begins to advance earlier, in partial agreement with changes in Ross Sea regional pack ice dynamics. Fast ice minimum extent within McMurdo Sound is significantly correlated with icebreaker arrival date as well as wind velocity. The potential impacts of changes in fast ice climatology on the local marine ecosystem are discussed.