Background/Aim: Due to the numerous beneficial effects of pomegranate that can be explained through its antioxidative effects, the aim of this study was to determine the antioxidant potential of pomegranate peel extract (PoPEx) prepared from pomegranate that was harvested in the southeast region of Herze-govina (Bosnia and Herzegovina), through in vitro and in vivo studies. Methods: In PoPEx total phenols, flavonoids, flavonols, flavan-3-ols and antho-cyanins content was determined, as well as several antioxidative assays, including 2,2 diphenyl-1-picrylhydrazyl assay (DPPH), 2,2'-azino bis(3-ethylbenzothi-azoline-6-sulphonic acid) assay (ABTS), iron (III)-2,4,6-tripyridyl-S-triazine complex assay (FRAP), reduction of copper(II) ions (CUPRAC) assay, Briggs-Rauscher oscillatory reactions, neutralisation of OH radicals and lipid peroxidation assay. In vivo studies were performed by administrating 100 mg/ kg of body weight of PoPEx to the rats by gavage for 7 days, after which the rats were euthanised and prooxidative parameters (thiobabrituric acid reactive substances-TBARS as an index of lipid peroxidation, nitrites-NO 2 , hydrogen peroxide-H 2 O 2 and superoxide anion radical O 2-) were determined in plasma, as well as antioxidative parameters (superoxide dismutase-SOD, reduced gluta-thione-GSH and catalase-CAT) in erythrocyte lysates. Results: High content of phenolic compounds was found in PoPEx, which resulted in high antioxidative potential in all in vitro tests performed. In vivo study showed that PoPEx administration caused a significant decrease in TBARS, NO 2-, as well as an increase in reduced glutathione (p < 0.05) in comparison to the control group, while H 2 O 2 and O 2 * showed a lowering trend and SOD and CAT showed an increasing trend in PoPEx group, but without statistical significance. Conclusion: PoPEx demonstrated high antioxidative capacity measured in vitro and in vivo and can be potentially used as a supplement treatment in the prevention of various inflammatory conditions.

The aim of this study was the determination of the antioxidant and antihyperglyce-mic effect of wild grown rosehip and pomegranate fruit extracts, and the determination of the polyphenolic content (total phenols, flavonoids, flavonols, flavan-3-ols and total and monomeric anthocyanins). The antioxidant activity of rosehip fruit in view of stable DPPH and ABTS radicals was higher comparing to pomegranate fruit, while according to the OH radical these two samples showed a similar effect (94.17 and 92.03 mg/mL). A dry rosehip fruit extract was found to have a 1.6 times more pronounced antihyperglycemic activity (1.42 mg/mL) compared to the dried pomegranate fruit extract (2.26 mg/mL). The content of total phenols of rosehip fruit was 8.75 mg GAE/g FPM and 22.01 mg GAE/g DPM , higher than the content of total phenols of pomegranate fruit (3.44 mg GAE/g FPM and 15.43 mg GAE/g DPM). The content of total phenols in rosehip and pomegranate fruits was higher than the content of total flavonoids, flavonols and flavan-3-ol and total and monomeric anthocyanins, which indicates that these components had the greatest impact on bioactivity of wiled grown fruits. Due to their bioactivity, the fruits of wild grown rosehip and pomegranate can be considered as potential functional food and food suitable for diabetics.