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A method has been developed for the fractionation of human erythrocyte membranes, using sodium deoxycholate, EDTA, differential ultracentrifugation, and DEAE-cellulose column chromatography. Serologic studies demonstrate the presence of ABO blood group antigens in a 50,000 g supernatant. This fraction is further purified on DEAE-cellulose, and in type AB ghost preparations results in the separation of A and B antigens. Phytohemagglutinin binding activity is also in the same supernatant and after DEAE chromatography is located in the fraction that exhibited A activity. Rh and MN activity is associated with a larger membrane fragment which is pelleted at 30,000 g. The S, s, and I antigens are present in a soluble form, and remain in the supernatant following centrifugation at 100,000 g.

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