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A yellow-to orange-pigmented, Gram-negative, rod-shaped, motile and non-spore-forming bacterium, strain FSW06-204d T , was isolated from subsurface water of the acidic bog lake, Lake Grosse Fuchskuhle (Brandenburg, Germany). Optimum growth of this strain occurred over a pH range from 5.5 to 6.0 and the growth rate strongly decreased at pH values above 6.5. In addition, the strain exhibited a low tolerance towards NaCl and grew only at a NaCl concentration of up to 0.5 %. 16S rRNA gene sequence analysis of strain FSW06-204d T showed the highest sequence similarity to Novosphingobium hassiacum W-51 T (96.7 %) and formed a distinct cluster with Novosphingobium nitrogenifigens DSM 19370 T (96.4 %) within the genus Novosphingobium. Strain FSW06-204d T shared a 21 bp signature gap with the latter species, a feature that is absent in all other members of the family Sphingomonadaceae. DNA-DNA hybridization of strain FSW06-204d T and N. nitrogenifigens DSM 19370 T showed a low relatedness value of 24 % (reciprocal: 39 %). The major respiratory quinone was ubiquinone Q-10 (91 %) and the predominant fatty acid was C 18 : 1 v7c (43.3 %). Two characteristic 2-hydroxy fatty acids, C 14 : 0 2-OH (8.1 %) and C 15 : 0 2-OH (6.5 %), were abundant. Polar lipids consisted mainly of phosphatidyldimethylethanolamine and phosphatidylethanolamine; however, only moderate amounts of sphingoglycolipids were present and phosphatidylcholine was lacking. Characterization by 16S rRNA gene sequence, physiological features, pigment analysis and polyamine, ubiquinone, polar lipid and fatty acid contents revealed that strain FSW06-204d T represents a novel species of the genus Novosphingobium within the class Alphaproteobacteria. The name Novosphingobium acidiphilum sp. nov. is proposed for this acidophilic and saltsensitive species with the type strain FSW06-204d T (5DSM 19966 T 5CCM 7496 T 5CCUG 55538 T ).

CCUG -Culture Collection University of Gö teborg, S-413 46 Gö teborg, SwedenStrain CCUG 50899, a Gram-negative, rod-shaped, non-spore-forming, motile bacterium isolated from industrial environment in Sweden and tentatively assigned to the species Ochrobactrum anthropi, was studied in order to clarify its taxonomic status. 16S rRNA gene sequence similarities placed the strain in the genus Ochrobactrum, sharing highest similarity with the type strains of Ochrobactrum rhizosphaerae (99.3 %), Ochrobactrum thiophenivorans (98.7 %), Ochrobactrum pseudogrignonense (98.6 %) and Ochrobactrum grignonense (98.5 %). The fatty acid profile of [O. anthropi] CCUG 50899 (major fatty acids C 18 : 1 v7c and C 19 : 0 cyclo v8c and presence of C 18 : 1 2-OH), the polar lipid profile (diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unknown aminolipids and an unknown phospholipid), the presence of the quinone system ubiquinone Q-10 and a polyamine pattern with the major compounds putrescine and spermidine and moderate amounts of sym-homospermidine supported its affiliation to the genus Ochrobactrum. DNA-DNA reassociation experiments with the type strains of its closest relatives O. rhizosphaerae, O. pseudogrignonense, O. thiophenivorans and O. grignonense demonstrated that [O. anthropi] CCUG 50899 should be placed in a novel species, which is distinguishable from related species by a set of biochemical traits. Based on these data, reclassification of [O. anthropi] CCUG 50899 as the type strain of a novel species appears to be justified. Hence, we describe a novel species to accommodate this strain, for which we propose the name Ochrobactrum pituitosum sp. nov. The type strain is CCUG 50899 T (5DSM 22207 T ).

A Gram-negative, rod-shaped, non-spore-forming organism, strain MSSRF26 T , was isolated from mangrove-associated wild rice in India. On the basis of 16S rRNA gene sequence similarities, strain MSSRF26T was shown to belong to the Alphaproteobacteria, most closely related toAltererythrobacter luteolus and Altererythrobacter epoxidivorans (96.1 and 95.9 % similarity to the respective type strains). Chemotaxonomic data [major ubiquinones Q-10 (91 %) and Q-9 (9 %); major polyamine spermidine, with putrescine, cadaverine and spermine detected only in trace amounts; major polar lipids phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and sphingoglycolipid; major fatty acid C 18 : 1 v7c and C 14 : 0 2-OH as hydroxylated fatty acid] supported the affiliation of MSSRF26 T to the genus Altererythrobacter.

Three Gram-positive, rod-shaped bacteria (strains CC-SBCK-209T, CC-12309T and CC-5209T) were isolated from the stalk of the edible mushroom Agaricus blazei grown in the laboratory. 16S rRNA gene sequence analysis indicated that all three isolates clearly belonged to the genus Microbacterium. Strains CC-SBCK-209T and CC-12309T were most related closely to the type strain of Microbacterium halotolerans (95.9 and 96.1 %16S rRNA gene sequence similarity, respectively). These two novel strains shared 97.9 % 16S rRNA gene sequence similarity. Levels of similarity to the type strains of all other recognized Microbacterium species were lower than 95.5 %. The third strain (CC-5209T) showed the highest 16S rRNA gene sequence similarity to the type strain of Microbacterium resistens (97.6 %); levels of similarity to the type strains of all other recognized Microbacterium species were lower than 96 %. The quinone systems of strains CC-SBCK-209T, CC-12309T and CC-5209T consisted of MK-11/MK-12, MK-11/MK-10 and MK-13 as major compounds, respectively. All three strains contained ornithine in their peptidoglycan. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The polyamine pattern consisted of spermidine and spermine as predominant components. Fatty acid profiles (anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 as major components) supported the affiliation of all three strains to the genus Microbacterium. The results of physiological and biochemical tests and DNA–DNA hybridization experiments allowed the clear phenotypic and genotypic differentiation of strains CC-SBCK-209T and CC-12309T from M. halotolerans and other closely related Microbacterium species. Strain CC-5209T could be differentiated clearly from M. resistens both genotypically and phenotypically. Based on these data, the novel strains are considered to represent three novel species of the genus Microbacterium. The names proposed for these organisms are Microbacterium agarici sp. nov. [type strain CC-SBCK-209T (=DSM 21798T=CCM 7686T)], Microbacterium humi sp. nov. [type strain CC-12309T (=DSM 21799T=CCM 7687T)] and Microbacterium pseudoresistens sp. nov. [type strain CC-5209T (=DSM 22185T=CCM 7688T)].