Neuronal networks in vitro are considered one of the most promising targets of research to assess potential electromagnetic field induced effects on neuronal functionality. A few exposure studies revealed there is currently no evidence of any adverse health effects caused by weak electromagnetic fields. Nevertheless, some published results are inconsistent. Particularly, doubts have been raised regarding possible athermal biological effects in the young brain during neuronal development. Therefore, we developed and characterized a flexible experimental setup based on a transverse electromagnetic waveguide, allowing controlled, reproducible exposure of developing neuronal networks in vitro. Measurement of S-parameters confirmed very good performance of the Stripline in the band of 800-1000 MHz. Simulations suggested a flexible positioning of cell culture dishes throughout a large exposure area, as specific absorption rate values were quite independent of their position (361.7 ± 11.4 mW/kg) at 1 W, 900 MHz. During exposure, thermal drift inside cellular medium did not exceed 0.1 K. Embryonic rat cortical neurons were cultivated on microelectrode array chips to non-invasively assess electrophysiological properties of electrogenic networks. Measurements were taken for several weeks, which attest to the experimental setup being a reliable system for long-term studies on developing neuronal tissue.
W październiku 2018 roku miałem okazję uczestniczyć w Światowym Synodzie Biskupów w Rzymie, który zgromadził się pod tytułem: „Młodzi, wiara i rozeznanie powołania”. Liczni biskupi i przełożeni zakonni z całego świata, doradcy – a przede wszystkim młodzi ludzie – spędzili miesiąc na dyskusji o tym, jak można dziś być i żyć Kościołem razem z młodymi ludźmi – i być ich towarzyszami w drodze do życia, do wiary i do odnalezienia swojego powołania.
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