Soybean oil has been supplemented with 10% (w/w) of ginger and turmeric powders derived from commercial products (GC—commercial ginger and TC—commercial turmeric), freeze-dried rhizomes (freeze-dried ginger (GR) and freeze-dried turmeric rhizome—TR) and peels (freeze-dried ginger peel (GP) and freeze-dried turmeric peel—TP) for developing a functional seasoning with great lipid stability for human consumption. The exhausted ginger and turmeric powders were also recovered and recycled two times to promote a more sustainable process. The antioxidant activity and oxidative stability of oil samples were evaluated respectively by spectrophotometric and Rancimat methods. Folin–Ciocalteu assay and HPLC analysis were also performed to quantify total polyphenols, ginger-derived 6-gingerol and 6-shogaol, and turmeric-derived curcumin. Their antioxidant activity as well as oxidative stability, which non-linearly decreased over cycles because of a strongly reduced phenolic extractability, linearly increased with increasing phenolic yields. Hence, ginger and turmeric can be proposed as healthy spices containing bioactive compounds to control lipid oxidation and improve oil stability. Moreover, the valorization of peels as eco-friendly source of natural antioxidants is a valid strategy for providing added-value to these agro-food wastes.
For centuries, extracts of Cynara cardunculus and the latex of Ficus carica have been used in cheesemaking, thanks to their proteases that clot milk casein. Since esterase activity is important to release odorous compounds in cheese, in this study we characterized the esterase activity of an extract of Cynara cardunculus and the latex of Ficus carica. The results, obtained by titration, spectrophotometric techniques, zymograms, and tests on agar plates, show the presence of esterase activity in the samples tested, with affinity towards the esters of short chain fatty acids, such as acetic, propionic, and butyric acid. In conclusion, this study provides a background information on esterases of two plants used in cheesemaking and represents a starting point for further investigations of their applications, not only in dairying but also in other industries involved in the processing and transformation of lipids.
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