No abstract

No abstract

Objective To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. Results The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. Conclusions In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.

Objective To evaluate the in vitro antibacterial and cytotoxic properties of the methanolic extract of Pelargonium peltatum (geranium) against Streptococcus mutans (ATCC 25175) and Streptococcus sanguinis (ATCC 10556). Methods Three extracts of P. peltatum were prepared using the leaf, stem, and root. Nine independent assays were prepared for each type of extract with chlorhexidine at 0.12% as the positive control. The agar diffusion method was performed to determine the antibacterial properties of each extract. The minimum inhibitory concentration (MIC) was determined using the microdilution method, and the cytotoxicity was analyzed by means of the MTT reduction test using a MDCK cell line. Results The root extract had the highest antibacterial effect with a mean result of (27.68 ± 0.97) mm and (30.80 ± 0.55) mm against S. mutans and S. sanguinis, respectively. The minimum inhibitory concentration for the leaf and root extracts was 250 mg/mL for S. mutans and 125 mg/mL for S. sanguinis. Cytotoxicity assays showed that both extracts had a low cytotoxicity at high concentrations. The cellular viability was highest for the root extract at 95.3% followed by the stem extract at 80.8% and finally the leaf extract with 75.4%. Conclusions These findings show the antibacterial properties of the methanolic extracts of P. pelargonium against S. mutans and S. sanguinis. These extracts were not cytotoxic at high concentrations.

Introducción: el uso constante de material punzocortante e instrumental rotatorio y la flora bacteriana de la cavidad oral del paciente representan un peligro latente de contagio en los estudiantes de odontología. El objetivo del estudio fue determinar la asociación entre los conocimientos y las actitudes sobre accidentes ocupacionales en prácticas clínicas de alumnos de odontología de una clínica universitaria de Lima (Perú). Materiales y métodos: estudio observacional, analítico de asociación y transversal. El universo incluyó 156 alumnos. Los conocimientos y las actitudes se evaluaron mediante una encuesta adaptada de 24 preguntas, previa validación interna por juicio de expertos. Se calcularon proporciones para las variables cualitativas y medidas de tendencia central (mediana) para la variable edad. De la misma manera, se usaron la prueba de chi-cuadrado para comparar las variables cualitativas y la prueba U de Mann-Whitney para comparar las variables cualitativas con la variable cuantitativa edad. Se aceptó una significancia de 0.05. Resultados: el 78.2 % de los participantes tuvo un conocimiento alto frente al manejo de accidentes ocupacionales; de la misma manera, el 72.4 % manifestó una actitud positiva. No se encontró asociación estadísticamente significativa (p > 0.05) entre las variables conocimientos y actitud. Por otro lado, el 69.1 % de los estudiantes sufrió al menos un accidente ocupacional durante sus prácticas clínicas, en su mayoría causado por el explorador (65.1 %). Conclusiones: no se encontró asociación significativa entre las variables conocimiento y actitud; sin embargo, una gran cantidad de estudiantes sufrieron al menos un accidente ocupacional.