Primary school teachers are often tasked with puberty/sexuality education for students who are undergoing sexual maturation at ever-earlier ages. This study explores the changing trajectories of the pre-service learning and teaching of primary school puberty/sexuality education at an urban university, including student-teachers' childhood learning, professional pre-service studies, and technological educational future. Biographical narratives and a theoretically based conceptual and diagnostic framework are used to analyse the views of fourth-year Bachelor of Education studentteachers about their own primary school puberty/sexuality education, their present university professional education for teaching these subjects, and their intentionality to teach them to future Grade 6 school students. Results show that these student-teachers intend to replicate their own primary school learning experiences. Pre-service professional education appears to have failed to operationalise the contemporary learning and teaching of puberty/sexuality education that would allow a new generation of more effective knowledge and pedagogies to be implemented.