The ArcLight observatory provides hourly continuous time series of
light regime data (intensity, spectral composition, and photoperiod)
from the Arctic, Svalbard at 79° N. Until now, no complete annual time
series of biologically relevant light has been provided from the high
Arctic due to insufficient sensitivity of commercial light sensors
during the Polar Night. We describe a camera system providing all-sky
images and the corresponding integrated spectral irradiance
(
E
P
A
R
) in energy or quanta units,
throughout a complete annual cycle. We present hourly–diel–annual
dynamics from 2017 to 2020 of irradiance and its relation to weather
conditions, sun and moon trajectories.
We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.
A cascade conical diffraction system consisting of three optically biaxial KGd(WO4)2 crystals is considered. The effect of left- and right-handed circularly polarized incident light on the ring patterns produced away from the focal image plane of the system, the plane in which the incident beam waist would be focused if the crystals were isotropic, is investigated. Images and intensity distributions for scaled distances (ζ values) of 2.75, 3.00, and 3.25 from the focal image plane are presented. A discrepancy between the patterns produced depending on the handedness of the incident beam is observed in agreement with the recent theoretical predictions.
Azimuthal and radial polarization states of light are used to produce conical diffraction (CD) from a KGd(WO4)2 crystal. The patterns produced in the ring plane in each case display marked differences than those seen when linearly polarized incident light is used, with the production of a splitting of the CD ring into two concentric rings of equal intensity. The free space evolution for each type of polarization state is also experimentally recorded and investigated. Comparison with theory shows agreement with the experimentally observed results.
Phase microscopy allows stain-free imaging of transparent biological samples. One technique, using the transport of intensity equation (TIE), can be performed without dedicated hardware by simply processing pairs of images taken at known spacings within the sample. The resulting TIE images are quantitative phase maps of unstained biological samples. Therefore, spatially resolved optical path length (OPL) information can also be determined. Using low-cost, opensource hardware, we applied the TIE to living algal cells to measure their effect on OPL. We obtained OPL values that were repeatable within species and differed by distinct amounts depending on the species being measured. We suggest TIE imaging as a method of discrimination between different algal species and, potentially, non-biological materials, based on refractive index/OPL. Potential applications in biogeochemical modelling and climate sciences are suggested.
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