Viscoelastic mechanical properties are frequently altered after tendon injuries and during recovery. Therefore, non-invasive measurements of shear viscoelastic properties may help evaluate tendon recovery and compare the effectiveness of different therapies. The objectives of this study are to present an elastography method to measure localized viscoelastic properties of tendon and to present initial results in healthy and injured human Achilles and semitendinosus tendons. The technique used an external actuator to generate the shear waves in the tendon at different frequencies and plane wave imaging to measure shear wave displacements. For each of the excitation frequencies, maps of direction specific wave speeds were calculated using Local Frequency Estimation. Maps of viscoelastic properties were obtained using a pixel wise curve-fit of wave speed and frequency. The method was validated by comparing measurements of wave speed in agarose gels to those obtained using magnetic resonance elastography. Measurements in human healthy Achilles tendons revealed a pronounced increase in wave speed as function of frequency that highlights the importance of tendon viscoelasticity. Additionally, the viscoelastic properties of the Achilles tendon were larger than those reported for other tissues. Measurements in a tendinopathic Achilles tendon showed that it is feasible to quantify local viscoeasltic properties. Similarly, measurement in the semitendinosus tendon showed a substantial differences in viscoelastic properties between the healthy and contralateral tendons. Consequently, this technique has the potential of evaluating localized changes in tendon viscoelastic properties due to injury and during recovery in a clinical setting.

Purpose The purpose of this study was to establish a relationship between the lengthening of the Achilles tendon post rupture and surgical repair to muscle activation patterns during walking in order to serve as a reference for post-surgical assessment. Method The Achilles tendon lengths were collected from 4 patients with an Achilles tendon rupture 6 and 12 month post-surgery along with 5 healthy controls via ultrasound. EMG was collected from the triceps surae muscles and tibialis anterior during over-ground walking. Results Achilles lengths at 6 and 12 months post-surgery were significantly longer (p < 0.05) on the involved side compared to the uninvolved side but there were no side to side differences in the healthy controls. The integrated EMG (iEMG) of the involved side was significantly higher than the uninvolved side in the lateral gastrocnemius at 6 months and for the medial gastrocnemius at 12 months in the patients with Achilles tendon rupture; no side to side difference was found in the healthy controls. The triceps surae muscles’ activations were fair to moderately correlated to the Achilles lengths (0.38 < r < 0.52). Conclusions The increased Achilles tendon length and iEMG from the triceps surae muscles indicate that loss of function is primarily caused by anatomical changes in the tendon and the appearance of muscle weakness is due to a lack of force transmission capability. This study indicates that when aiming for full return of function and strength an important treatment goal appears to be to minimize tendon elongation. Level of evidence Prognostic prospective case series. Level IV.

Changes in tendon viscoelastic properties are observed after injuries and during healing as a product of altered composition and structure. Continuous Shear Wave Elastography is a new technique measuring viscoelastic properties of soft tissues using external shear waves. Tendon has not been studied with this technique, therefore, the aims of this study were to establish the range of shear and viscosity moduli in healthy Achilles tendons, determine bilateral differences of these parameters and explore correlations of viscoelasticity to plantar flexion strength and tendon area. Continuous Shear Wave Elastography was performed over the free portion of both Achilles tendons from 29 subjects. Isometric plantar flexion strength and cross sectional area were measured. The average shear and viscous moduli was 83.2kPa and 141.0Pa-s, respectively. No correlations existed between the shear or viscous modulus and area or strength. This indicates that viscoelastic properties can be considered novel, independent biomarkers. The shear and viscosity moduli were bilaterally equivalent (p=0.013,0.017) which allows determining pathologies through side-to-side deviations. The average bilateral coefficient of variation was 7.2% and 9.4% for shear and viscosity modulus, respectively. The viscoelastic properties of the Achilles tendon may provide an unbiased, non-subjective rating system of tendon recovery and optimizing treatment strategies.

Background:Semitendinosus/gracilis (STG) tendon autograft has been used effectively for restoring knee stability after anterior cruciate ligament (ACL) rupture. Though ACL reconstruction with STG autograft is an effective surgical technique for return to sports, short-term hamstring strength asymmetries exist after surgery. Although imaging evidence has demonstrated regrowth and reorganization of the semitendinosus (ST) tendon, no studies show whether the regrowth is associated with residual muscle function. Continuous shear wave elastography (cSWE) using an external actuator and high–frame rate ultrasound is a promising technique for evaluating the mechanical properties of regrown tendons in vivo.Purpose:To demonstrate recovery of the mechanical properties of the hamstring tendons after ACL reconstruction using an STG tendon autograft.Study Design:Case series; Level of evidence, 4.Methods:Thirteen patients underwent an STG autograft reconstruction after ACL rupture. Regrowth of the ST tendon was confirmed via b-mode ultrasound between 6 and 24 months postreconstruction. The shear elastic (µ 1) and viscosity moduli (µ 2) of the ST tendons were ascertained through cSWE using an external actuator and high–frame rate ultrasound.Results:Significant differences in both shear elastic (129.4 vs 73.0 kPa) and viscous moduli (192.6 vs 114.3 Pa·s) existed bilaterally for uninvolved and involved limbs, respectively. Additionally, a positive correlation between time postoperative and shear elasticity was observed (r = 0.60). More than 12 months were required for patients to regain a large percentage of the tendon’s mechanical properties compared with the contralateral side (µ 1, 80.6% at >12 months vs 39.9%; µ 2, 78.7% at >12 months vs 46.0%).Conclusion:The imaging and elastography data demonstrate tendon regrowth and recovery of functional biomechanical properties with time. The elastic modulus of the recovered tendon indicates the ability to transmit muscle force across the joint and recovery of semitendinosus function after its use for an ACL graft.