This is the unspecified version of the paper.This version of the publication may differ from the final published version. Abstract: The dynamic aberrations introduced by the human tear film are studied by measuring the topography of the tear film surface on 14 subjects using a curvature sensing setup. The RMS wavefront error variation of the data obtained is presented showing the non-negligible contribution of the tear film to overall eye aberrations. The tear film wavefronts are decomposed in their constituent Zernike terms, showing stronger contributions from 4th order terms and terms with vertical symmetry, and the temporal behaviour of these aberrations is analysed. Permanent

This is the unspecified version of the paper.This version of the publication may differ from the final published version. Abstract: Structured illumination microscopy applied to in-vivo retinal imaging has the potential to provide a low-cost and powerful diagnostic tool for retinal disease. In this paper the key parameters that affect performance in structured illumination ophthalmoscopy are studied theoretically. These include the number of images that need to be acquired in order to generate a sectioned image, which is affected by the non-stationary nature of the retina during acquisition, the choice of spatial frequency of the illuminating sinusoid, the effect of typical ocular aberrations on axial resolution and the nature of the sinusoidal pattern produced by the illumination system. The results indicate that structured illumination ophthalmoscopy can be a robust technique for achieving axial sectioning in retinal imaging without the need for complex optical systems. Permanent repository link

Current implementations of structured illumination microscopy for depth-resolved (three-dimensional) imaging have limitations that restrict its use; specifically, they are not applicable to non-stationary objects imaged with relatively poor condenser optics and in non-fluorescent mode. This includes in-vivo retinal imaging. A novel implementation of structured illumination microscopy is presented that overcomes these issues. A threewavelength illumination technique is used to obtain the three sub-images required for structured illumination simultaneously rather than sequentially, enabling use on non-stationary objects. An illumination method is presented that produces an incoherent pattern through interference, bypassing the limitations imposed by the aberrations of the condenser lens and thus enabling axial sectioning in non-fluorescent imaging. The application to retinal imaging can lead to a device with similar sectioning capabilities to confocal microscopy without the optical complexity (and cost) required for scanning systems.

Citation:Gowree, E. R., Atkin, C. J. and Gruppetta, S. (2015). A simple digital-optical system to improve accuracy of hot-wire measurements. MEASUREMENT SCIENCE and TECHNOLOGY, 26(9), This is the accepted version of the paper.This version of the publication may differ from the final published version. Permanent AbstractA high precision traverse mechanism with micro-resolution was designed to capture accurately the velocity profile of the very thin turbulent attachment line on a swept body. To ensure that the traverse mechanism could position the hot wire reliably, a simple digital optical system was designed to check the performance of the traverse by measuring the displacement of the hot wire: a vertical displacement of 2.4µm was achievable and this could be further reduced to 0.6µm using micro-stepping. Due to the simplicity of the set-up it was equally useful for probe wall positioning and the velocity profiles captured clearly demonstrated that the optical set-up helped in resolving the near wall flow more accurately, regardless of the thinness of the boundary layer. The captured data compare well with the results from similar investigations, with arguably higher precision achieved.

No abstract