Co-extirpation among host-affiliate species is thought to be a leading cause of biodiversity loss worldwide. Freshwater mussels (Unionida) are at risk globally and face many threats to survival, including limited access to viable host fish required to complete their life history. We examine the relationship between the common eastern elliptio mussel (Elliptio complanata) and its migratory host fish the American eel (Anguilla rostrata), whose distribution in the Chesapeake Bay watershed is limited, in part, by dams. We examined population demographics of E. complanata across locations in the Chesapeake Bay watershed, primarily in the Susquehanna River in the absence of American eels, and conducted experimental restocking of eels to assess potential impacts on mussel recruitment. Compared to surveys completed~20 yr prior, E. complanata could be experiencing declines at several historically abundant sites. These sites also had extremely limited evidence of recruitment. Restoration of host fish improved recruitment, but results were not equivalent between stocking sites, indicating that host reintroduction alone may not be fully effective in reestablishing mussel populations. One site where eels were introduced (Pine Creek, Tioga County, Pennsylvania, USA) experienced an increase from 0 juveniles found during quantitative surveys prior to eel stocking to 151 (21% of individuals collected during quantitative surveys) E. complanata juveniles found four years after stocking. A second site (Buffalo Creek, Union County, Pennsylvania) experienced a more moderate increase from 2 to 7 juveniles found during 2010 and 2014 quantitative surveys, respectively. Continued examination of other potential interacting factors affecting recruitment, including water quality or habitat conditions, is necessary to target favorable sites for successful restoration.

American Eel Anguilla rostrata populations along the Atlantic coast of the United States have been in decline over the past several decades. One suggested cause of the decline is construction of barriers that block access to upstream tributaries where they can spend a significant portion of their lives. Success of reintroduction efforts above barriers has rarely been evaluated. Within the Susquehanna River (Chesapeake Bay watershed), over 1 million eels were released above four major downstream barriers in the past decade. We used backpack electrofishing and tagging to monitor growth, sexual differentiation, and population density of reintroduced eels in Buffalo Creek, a tributary to the Susquehanna River (Pennsylvania). From 2012 to 2019, we caught over 2,000 individuals, tagged more than 1,800, and recaptured 229. Recaptured eels provided insight into growth, sexual differentiation, and movement. Nearly 99% of recaptures remained near stocking locations. The average growth rate was 47.8 mm/y and ranged between −5.8 and 116.0 mm/y. Females generally grew significantly faster than males, and growth rates of several females exceeded 100 mm/y, a rate typically associated with estuarine residents. The population density within stocking sites was over 2,300 eels/km, roughly four times higher than Susquehanna River tributaries below the most downstream dam, and exceeded the target stocking goal of 529 eels/km. While we caught most eels in areas sampled near stocking locations, we captured some eels in smaller upstream tributaries away from stocking locations. Our study is the first to examine how reintroduced eels grow following stocking above four major dams on the Susquehanna River. We suggest that managers considering moving eels above blockages account for release location and density to achieve desired benefits to the overall population.