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The binding of the antagonists N-(8-aminooctyl)-5-iodonaphthalene-1-sulfonamide (J-8) and trifluoperazine (TFP) to intact calcium-saturated bovine calmodulin (CaM) and also of J-8 to the C-terminal domain (tr2c) has been investigated. Using a combination of NMR methods, including NOESY data, mobility measurements, and chemical shift and line-shape analysis, we show that the primary interaction between J-8 and tr2c is between the naphthalene ring of the antagonist and the hydrophobic pocket of the protein, similar to the binding of the hydrophobic side-chain residues of calmodulin target peptides. Comparison of the mobility of the drug, the intensity and pattern of intermolecular NOESY cross-peaks, and chemical shift changes shows that there is no significant change in the binding mode in J-8‚CaM compared to J-8‚tr2c, with one molecule binding to each domain. In particular, we find that the mobility of the aliphatic amino "tail" of J-8 remains highly mobile in both systems. This contrasts with the notion that the tail may bridge between the two domains to give a "globular" form of CaM. We also show that TFP induces very similar shift changes to J-8 and that the stoichiometry of the major binding event in all three cases is one drug molecule per domain. It also appears that secondary binding sites for the drug molecules are present in all three systems.

This chapter describes research on team member contributions to overall team effectiveness, and various applications of this research to developing and assessing teamwork by students on team and group projects and assignments.

The purpose of this research was to develop and validate a new measure of undergraduate student cynicism-the Cynical Attitudes Toward College Scale (CATCS). In Study 1, the authors split data collected from 1,049 students representing 3 diverse college populations into development and confirmation samples. Using confirmatory factor analysis, the authors (a) developed an 18-item measurement model reflecting 4 reliable dimensions of student cynicism-policy, academic, social, and institutional; (b) demonstrated cross-sample generalizability; and (c) established convergent and discriminant validity. In Study 2, the authors showed the CATCS's ability to discriminate among diverse groups of students predicted to differ on the 4 subscales as well as to predict future academic outcomes. Practical applications of the CATCS are discussed.

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