Carfentrazone is a broadleaf weed control herbicide that is also used for control of silvery-thread moss (Bryum argenteum) in creeping bentgrass (Agrostis stolonifera) putting greens. Field studies were initiated in June 2006 and May 2007 to evaluate silvery-thread moss control with carfentrazone alone, carfentrazone applied with nitrogen (N) and/or topdressing (TD), N alone, TD alone, and mancozeb plus copper hydroxide. All treatments except for mancozeb plus copper hydroxide and the non-treated control reduced silvery-thread moss populations 16 weeks after initial treatment. Carfentrazone applied alone and carfentrazone followed by N decreased silvery-thread moss populations by 39%. Carfentrazone followed by TD and carfentrazone followed by N + TD decreased silvery-thread moss populations by 73% and 66%, respectively. These data indicate the importance of using cultural practices to control silvery-thread moss on creeping bentgrass putting greens.

Nutrient and sediment runoff from newly constructed levee embankments pose a threat to water quality during soft armor vegetation establishment. Research was initiated in 2008 and 2009 to evaluate the effect of bermudagrass ( L.) coverage and N source on nutrient and sediment runoff from levee embankments during establishment. Bermudagrass plots were seeded at 195.3 kg pure live seed ha and fertilized at 50 kg N ha using a water-soluble N source, urea or NH-NO, or slow-release N source, S-coated urea (SCU) or urea formaldehyde (UF), with controls unfertilized. Vegetative cover percentage, time until the onset of runoff, runoff volume, and total solids (TS), NO-N, and NH-N concentrations were measured from simulated and natural rainfall events for 70 d in 2008 and 56 d in 2009. Bermudagrass at 90% grass cover delayed the onset of runoff an additional 441 to 538 s and reduced runoff volumes 74 to 84% of that exhibited at 10% grass cover. Nitrogen fertilizers did not accelerate bermudagrass growth sufficiently, however, to reduce TS loading compared with unfertilized bermudagrass in either year of the study. The application of urea and SCU resulted in cumulative N losses of 2.45 and 3.13 kg ha compared with 1.59 kg ha from the unfertilized bermudagrass in 2008, and 1.73 kg ha from NH-NO vs. 0.24 kg ha from controls in 2009. Only UF increased bermudagrass establishment without increasing cumulative N losses compared with unfertilized bermudagrass. Therefore, the benefit of greater erosion and runoff resistance expected from N-accelerated vegetative growth did not occur but had the unintended consequence of higher N losses when water-soluble N and SCU fertilizers were applied.

Torpedograss infestation in centipedegrass has reduced centipedegrass quality in home lawns along the Gulf Coast. This study was conducted to evaluate three selective postemergence herbicides. Field trials were conducted at two sites in Louisiana to evaluate quinclorac, sethoxydim, and clethodim applied once or sequentially every 4 wk for selective torpedograss control in centipedegrass turf. Herbicides were applied to mixed stands of torpedograss/centipedegrass at two locations in Louisiana and evaluated for changes in torpedograss coverage and centipedegrass injury every 2 wk for 16 wk. All herbicides controlled torpedograss more with each sequential application. Sethoxydim and clethodim applied three times reduced torpedograss cover 84 and 87%, respectively, and more than quinclorac 12 wk after initial treatment (WAIT). Increasing clethodim or sethoxydim rates did not improve torpedograss control. Torpedograss regrowth occurred within weeks after final herbicide applications regardless of herbicide. Only multiple clethodim applied at twice manufacturer's labeled rate or quinclorac applications resulted in commercially unacceptable (> 25%) injury to centipedegrass. Multiple sethoxydim or clethodim applications at 0.32 kg ha−1or 0.30 kg ha−1every 4 wk reduced torpedograss competitiveness in centipedegrass; however, multiple applications for more than 1 yr might be necessary to achieve torpedograss control.

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Huanglongbing (HLB) is considered the most destructive disease in Citrus production and threatens the future of the industry. Microbial-derived defense elicitors have gained recognition for their role in plant defense priming. This work assessed a 5% (V/V) microbial fermentation application (MFA) and its role in the elicitation of defense responses in HLB-infected Citrus sinensis trees following a foliar application with a pump sprayer. Using a PCR detection method, HLB infection levels were monitored in healthy and infected trees for 20months. Nutrient analysis assessed N, P, K, Ca, Mg, Mn, Zn, Fe, B, and Cu concentrations in the trees. MFA significantly increased Cu concentrations in treated trees and resulted in the stabilization of disease index (DI) in infected trees. Initial real-time qPCR analysis of defense-associated genes showed a significant increase in pathogenesis-related protein 2 (PR2) and phenylalanine ammonia lyase (PAL) gene expression in healthy and HLB-infected trees in response to MFA. Gene expression of PR2 and PAL peaked 6h post-microbial fermentation application during an 8-h sampling period. A transcriptomic assessment using GeneChip microarray of the hour 6 samples revealed differential expression of 565 genes when MFA was applied to healthy trees and 909 genes when applied infected citrus trees when compared to their respective controls. There were 403 uniquely differentially expressed genes in response to MFA following an intersectional analysis of both healthy and infected citrus trees. The transcriptomic analysis revealed that several genes associated with plant development, growth, and defense were upregulated in response to MFA, including multiple PR genes, lignin formation genes, ROS-related genes, hormone synthases, and hormone regulators. This study provides further evidence that MFA may play an important role as a plant elicitor in an integrated pest management strategy in citrus and other agronomically important crops.