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Two colorimetric broth microdilution antifungal susceptibility tests were compared, Sensititre YeastOne and MICRONAUT-AM for nine antifungal agents. One hundred clinical Candida isolates were tested, representing a realistic population for susceptibility testing in daily practice. The reproducibility characteristics were comparable. Only for fluconazole, caspofungin, 5-flucytosine and amphotericin B, an essential agreement of ≥90% could be demonstrated. Sensititre minimal inhibitory concentrations (MICs) were systematically higher than MICRONAUT MICs for all antifungals, except for itraconazole. CLSI clinical breakpoints (CBPs) and epidemiological cut-off values (ECVs) were used for Sensititre MICs while for MICRONAUT the EUCAST CBPs and ECVs were used. Only fluconazole, micafungin, and amphotericin B had a categorical agreement of ≥90%. For fluconazole, micafungin, and amphotericin B the susceptibility proportions were comparable. Susceptibility proportion of posaconazole and voriconazole was higher using the MICRONAUT system. For itraconazole and anidulafungin, the susceptibility proportion was higher using Sensititre. It was not possible to determine the true MIC values or the correctness of a S/I/R result since both commercial systems were validated against a different reference method. These findings show that there is a significant variability in susceptibility pattern and consequently on use of antifungals in daily practice, depending on the choice of commercial system.

A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715 T , was isolated from the blood of a patient with Fournier's gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715 T was shown to belong to the genus Atopobium and was most closely related to Atopobium minutum (95 % similarity). The results of 16S rRNA-gene-based phylogenetic analysis, cellular fatty acid analysis and differential biochemical tests, showed that strain HHRM1715 T represented a novel species of the genus Atopobium. We therefore describe Atopobium deltae sp. nov. with HHRM1715 T (5LMG 27987 T 5CCUG 65171 T ) as the type strain and propose an emended description of the genus Atopobium with regard to the DNA G+C content.The genus Atopobium was introduced by Collins & Wallbanks (1992) to reclassify species formerly designated Lactobacillus minutus (Hauduroy et al., 1937), Lactobacillus rimae (Olsen et al., 1991) and Streptococcus parvulus (Weinberg et al., 1937). In 1999, Eubacterium fossor (Bailey & Love, 1986) and a newly described species, Atopobium vaginae (Rodriguez Jovita et al., 1999), were added to the genus (Kageyama et al., 1999).At the time of writing, the genus comprises five species, i.e. A. vaginae (type strain CCUG 38953 T ), A. rimae (ATCC 49626 T ), A. parvulum (ATCC 33793 T ), A. fossor (NCTC 11919 T ) and A. minutum (ATCC 33267 T ), with the last being the type species (Collins & Wallbanks, 1992), and is placed together with the genus Olsenella under the newly proposed family Atopobiaceae (Gupta et al., 2013). Olsenella umbonata (Kraatz et al., 2011) is the closest phylogenetic relative of the genus.Here, we report on the isolation of a strain, HHRM1715 T , from the blood of a patient with Fournier's gangrene, complicated with sepsis. Sequencing of the 16S rRNA gene, fatty acid analysis, matrix assisted laser desorption/ ionization time-of-flight (MALDI-TOF) MS analysis and biochemical tests showed that this isolate represented a novel species of the genus Atopobium, which we propose to name Atopobium deltae sp. nov. Strain HHRM1715 T was isolated from the blood of a patient with Fournier's gangrene, complicated by sepsis (Oyaert et al., 2014). After incubation of two anaerobic blood culture bottles (BACTEC FX, Becton Dickinson) for 83 h at 35 u C, the blood culture bottles yielded short, rod-shaped, Gram-stain-positive, catalase-negative bacteria. Neither a commercially available Rapid ID 32A test (bioMérieux), producing a presumptive identification of a member of the genus Bacillus with poor probability (53.1 %), nor MALDI-TOF MS (database MBT-BDAL-5627) (Bruker), giving an identification score of 1.317 for Sphingobium chlorophenolicum, resulted in identification of the strain.Subsequently, the nearly complete 16S rRNA gene was sequenced as described by Cools et al. (2013) (1368 bases) and compared with the publicly available bacterial 16S rRNA gene sequences deposited in GenBank using the Basic Local Alignment Search Tool (BLAST) software (www.ncbi.nlm.ni...

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