Group A rotavirus (RVA) infections form a major public health problem, especially in low-income countries like the Democratic Republic of the Congo (COD). However, limited data on RVA diversity is available from sub-Saharan Africa in general and the COD in particular. Therefore, the first aim of this study was to determine the genetic diversity of 99 RVAs detected during 2007–2010 in Kisangani, COD. The predominant G-type was G1 (39%) and the most predominant P-type was P[6] (53%). A total of eight different G/P-combinations were found: G1P[8] (28%), G8P[6] (26%), G2P[4] (14%), G12P[6] (13%), G1P[6] (11%), G9P[8] (4%), G4P[6] (2%) and G8P[4] (1%). The second aim of this study was to gain insight into the diversity of P[6] RVA strains in the COD. Therefore, we selected five P[6] RVA strains in combination with the G1, G4, G8 (2x) or G12 genotype for complete genome analysis. Complete genome analysis showed that the genetic background of the G1P[6] and G12P[6] strains was entirely composed of genotype 1 (Wa-like), while the segments of the two G8P[6] strains were identified as genotype 2 (DS-1-like). Interestingly, all four strains possessed a NSP4 gene of animal origin. The analyzed G4P[6] RVA strain was found to possess the unusual G4-P[6]-I1-R1-C1-M1-A1-N1-T7-E1-H1 constellation. Although the majority of its genes (if not all), were presumably of porcine origin, this strain was able to cause gastro-enteritis in humans. The high prevalence of unusual RVA strains in the COD highlights the need for continued surveillance of RVA diversity in the COD. These results also underline the importance of complete genetic characterization of RVA strains and indicate that reassortments and interspecies transmission among human and animal RVAs strains occur regularly. Based on these data, RVA vaccines will be challenged with a wide variety of different RVA strain types in the COD.

This paper describes the development of a microgasturbine with a rotor diameter of 20 mm. The target electrical power output lies around 1 kW. The total system fits in a cylinder with a diameter of 95 mm and a length of 120 mm. The system contains the same components as a large gasturbine generator: compressor, recuperator, combustion chamber, turbine, and electrical generator. Major challenges are the high rotational speed (500 000 r/min), high turbine inlet temperature (1200 K), and the efficiency of the components. Because of the small dimensions, the flow through compressor and turbine is characterized by relatively low Reynolds numbers. The higher flow losses and inherently lower efficiency require a higher blade tip speed (524 m/s) than for large turbines (300-400 m/s). To minimize wear and frictional losses, the rotor is mounted on aerodynamic bearings. To withstand the high centrifugal stresses, a high-strength steel is used for compressor and shaft. The turbine is made of a Si3N4-TiN ceramic composite to withstand the combination of elevated stress and temperature.

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