Different levels of dried Jerusalem artichoke were fed to entire male pigs 1 week before slaughter. The objective was to investigate the effect on skatole level in the hindgut and in adipose tissue, as well as the effect on microflora and short-chain fatty acids (SCFA) in the hindgut. Five experimental groups (n 5 11) were given different dietary treatments 7 days before slaughtering: negative control (basal diet), positive control (basal diet 1 9% chicory-inulin), basal diet 1 4.1% Jerusalem artichoke, basal diet 1 8.1% Jerusalem artichoke and basal diet 1 12.2% Jerusalem artichoke. Samples from colon, rectum, faeces and adipose tissue were collected. Effect of dietary treatment on skatole, indole and androstenone levels in adipose tissue and on skatole, indole, pH, dry matter (DM), microbiota and SCFA in the hindgut was evaluated. Feeding increasing levels of Jerusalem artichoke to entire male pigs reduced skatole in digesta from colon and in faeces (linear, P , 0.01). There was also a tendency towards a decreased level of skatole in adipose tissue (linear, P 5 0.06). Feeding Jerusalem artichoke decreased DM content in colon and faeces and pH in colon (linear, P , 0.01). Increasing levels of Jerusalem artichoke resulted in a reduced level of Clostridium perfringens in both colon and rectum (linear, P , 0.05) and a tendency towards decreased levels of enterobacteria in colon (linear, P 5 0.05). Further, there was an increase in total amount of SCFA (linear, P , 0.05), acetic acid (linear, P , 0.05) and valerianic acid (linear, P , 0.01) in faeces. In conclusion, adding dried Jerusalem artichoke to diets for entire male pigs 1 week before slaughter resulted in a dose-dependent decrease in skatole levels in the hindgut and adipose tissue. The reduced skatole levels might be related to the decrease in C. perfringens and the increase in SCFA with subsequent reduction in pH.

Supplementation of feed for bulls with selenium (+50%), vitamin D (+300%), vitamin E (+825%), vitamin K (+325%) and omega-3 fatty acids (+120%) affected beef nutrient composition. Twelve bulls (½ year old) were randomly allocated to two dietary treatments; control (Con) or supplemented (Sup), and fed 170 days pre-slaughter at an amount of 1% of body weight. Daily gain and feed efficiency were equal in the two groups. Homogenate meat from left forequarter in the Sup group contained more selenium (+26%), vitamin MK4 (+123%), D (+197%), E (+318%), and had lower omega-6/omega-3 ratio (-24%) compared to Con meat. Sup meat fulfilled the requirements to be labelled by health claims and nutrient claims as: "A food item containing a significant amount of selenium, vitamin K and vitamin D". We suggest supplementation of cattle rations during the finishing period as a strategy to increase meat content of specific nutrients important to human health.

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This study compared apparent ileal and total tract digestibility of macronutrients and amino acids in blue foxes (Alopex lagopus) fed dry extruded diets. The control diet contained fish meal as the main protein source, while in the other diets 50% of the fish meal protein was replaced by not de-hulled solvent-extracted soybean meal, meat meal or bacterial protein meal. Ileal digestibility was obtained with animals modified with ileorectal anastomosis (IRA method). There were no significant differences in the average digestibility of CP, Lys, Trp, Val, Ala or Glu between IRA modified and intact animals. Average ileal digestibilities of His, Thr, Asp, Cys, Gly, Hyp, Pro, Ser, starch and total carbohydrates (CHO) were significantly lower compared with total tract values. Average ileal digestibility of Arg, Ile, Leu, Met, Phe, Tyr and crude fat was significantly higher than total tract digestibility. Digestibility measured in IRA modified animals showed less variability compared with values from intact animals. There were significant differences among diets in ileal as well as total tract digestibility of orude protein, most amino acids, starch and CHO, and in ileal digestibility of crude fat. Ileal crude protein digestibility of the diets ranged from 81.0-86.4% and total tract digestibility from 82.5-86.4%. Ileal amino acid digestibilities ranged from 70.1 (Asp) to 93.3% (Arg) and total tract values ranged from 72.8 (Cys) to 92.2% (Arg). Both ileal and total tract digestibilities of crude protein and most amino acids were significantly lower for diets containing meat meal or bacterial protein meal compared with the control fish meal-based diet and the diet with soybean meal. Although ileal digestibility may be more accurate than total tract digestibility in estimating the protein and amino acid supply in blue foxes, total tract digestibility may be acceptable because of numerically small differences between ileal and total tract digestibilities for protein and most amino acids. It was further concluded that ileal digestibilities of starch and CHO in the blue fox are lower than corresponding total tract digestibilities.

Major nutrient and amino acid digestibilities were evaluated in experiments with dogs (Canis familiaris), blue foxes (Alopex lagopus) and mink (Mustela vison) using seven extruded diets containing different protein sources. Four dogs (German shepherd), four blue foxes and four mink were offered each of the experimental diets to determine apparent total tract digestibility. Average digestibility of crude protein (CP) in blue foxes and mink was similar, and lower than the corresponding value in dogs (0·834 and 0·831 v. 0·864) (P < 0·001). CP digestibility in blue foxes showed significant correlations with dogs and mink (P < 0·05), while no significant correlation was observed between dogs and mink. Average digestibility of most amino acids was significantly lower in blue foxes than in dogs (P < 0·001), whereas mink digested most essential amino acids at the same level as did dogs. Most non-essential amino acids were digested at a significantly lower rate in the mink than in the dog and the blue fox (P < 0·001). There were significant correlations in the digestibility of most amino acids between dogs and blue foxes, and between blue foxes and mink (P < 0·05). Correlations were significant between dogs and mink for the digestibility of most essential amino acids (P < 0·05) but not significant (P > 0·05) for several non-essential amino acids. Average digestibility of crude fat was high and similar among the species studied: 0·968, 0·967 and 0·959 in dogs, blue foxes and mink, respectively. Average digestibility of starch ranged from 0·971 in mink to 0·998 in dogs; significantly higher in dogs than in blue foxes and mink, and significantly higher in blue foxes than in mink (P < 0·001). It was concluded that digestibility determined with mink, blue foxes or dogs may be used to predict digestibilities in the other investigated species. However, both the characteristics of the protein sources as well as the individual amino acid studied will influence the difference in digestibility among the species.