Insulin receptor (IR) proteins were essential intracellular signaling peptides in the insulin action cascade. Insulin receptor substrate proteins (IRS-1and IRS-2) serve and regulate the insulin level in the normal insulin action. The broad role of IRS-1 and IRS-2 in cell growth and survival reveals a common regulatory pathway linking development, somatic growth, fertility, neuronal proliferation, and aging to the core mechanisms used by vertebrates for nutrient sensing. Such type of proteins were cyclic adenosine monophosphate-activated protein kinase, this proteins play a key role in the insulin response and regulation. Type -2 Diabetes mellitus occurs during prolonged periods of peripheral insulin resistance due to inactivation of IRS proteins. The compounds isolated from the medicinal plants were safer than synthetic drugs and possess high bio activity. In the present study, four compounds were elucidated from fruits of Helicteres isora. The elucidated compounds were evaluated for the antidiabetic activity using in silico docking study. The receptor was analyzed for the active site and pocket finder tools. The aminoacids such as Phenylalanine, Lysine, Glutamic acid and Asparigine were predicted as active site binding residues. Docking studies were done through Autodock 4 software. All the compounds from fruits of Helicteres isora showed good docking profiles with AMP Kinase, except compound-3 (1,2,3,4-tetrahydro-1,5,6,8-tetramethyl-7-(2-methylprop-1-enylnaphthalene-4-ylpivalate). Finally the result from the study demonstrates that the HS-1, HS-2 and HS-4 posses potent anti diabetic activity against type-2 diabetes mellitus through drug action on AMP kinase cascade system.

IntroductionDiabetes mellitus is a metabolic disorder caused due to the deficiency of insulin. This hormone controls the body processes and the metabolites such as glucose, protein, and fats. When the insulin synthesis is not sufficient, it cannot metabolize the carbohydrates and the other intermediately metabolites (fats and protein). This leads to accumulation high glucose in blood and urine. It is characterized as hyperglycemia and poly urea. In chronic the elevated levels of sugar can cause diabetic coma and ends in death [1]. Allopathic medicines were high in the cost, posse's side effects and the relief was not sustained permanently. The management and control of diabetes has continued to be challenging on type 2 diabetes mellitus.Yogic exercises cause the muscles to absorb the glucose in the blood, thus the blood sugar level was reduced in diabetic patients [2]. Yogic exercises trigger the pancreas and liver to function very effectively, leads to regulate the blood sugar level in homeostasis by induction of insulin secretions [3]. Hence the muscular movements play important role in bringing down the blood sugar levels. Meditations and asana significantly lead the healthy functioning of the internal organs of the body. Combined therapy such as naturopathy healing with herbal and natural product [4] treating for various diseases paved a significant recovery and improvement in the management of diabetes mellitus. The present case report emphasizes the efficacy of H. isora fruit concoction in type-2 diabetic patients practicing yoga and exercise. Case ReportType-2 Diabetic patients aged ranges from 40-60 years old volunteers were chosen for this case study. The diabetic patients were practicing yoga and exercise in the Osho meditation center and Simplified kundalini yoga center. The case study was experimentally grouped into four groups. The patients practicing Osho meditation and exercise were administered orally with H.isora concoction 5 mg/5 ml AbstractBackground: Diabetes mellitus patients thrive very hard to reduce their glucose levels by using various hypoglycemic agents. Many hypoglycemic agents cause side effects and fail to reduce glucose level, when they use for a long time. Folklore natural medicines will successfully act on many diseases without causing any side effects when it was consumes prolonged. Patients practicing yoga and exercise with the traditional medicines contribute a significant efficacy in management of diabetes in very short period.

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