Hybrid grouper (Epinephelus fuscoguttatus ♀ χ E. lanceolatus ♂) that is well known as “cantang” has a good prospect for aquaculture due to its high economic value, fast growing and good acceptance to compound feed. However, information on feed specification for this grouper is still limited. Therefore, this research aimed to find out the good proportions of protein, lipid and carbohydrate in feed for hybrid grouper. Six experimental diets were formulated with protein contents of 48, 44 and 40%, which then combined with lipid contents of 9-17% and carbohydrate contents of 15-32%. Juvenile hybrid grouper of 4.8 ± 0.6 g body weight were stocked into 24 fiber tanks, 400 L in volume, with a density of 40 fish/tank. Fish were fed experimental diet twice every day for 56 days. Resuls of the experiment showed specific growth rate (SGR) of fish fed 48% protein diet was not significantly different with SGR of fish fed diet containing 44% protein, 14% lipid, 15% carbohydrate and SGR of fish fed diet containing 40% protein, 16% lipid, 15% carbohydrate. The best protein efficiency ratio and protein retention were achieved in fish fed diet containing 44% protein, 14% lipid and 15% carbohydrate as well as in fish fed diet containing 40% protein, 16% lipid and 15% carbohydrate. This result suggested feed containing 44% protein, 14% lipid and 15% carbohydrate or feed containing 40% protein, 16% lipid and 15% carbohydrates was effective in increasing dietary protein efficiency and support good growth for juvenile hybrid grouper cantang.

To date, lobster aquaculture relies on fresh food for feeding; however, the availability of fresh food is limited and its excessive use causes environmental degradation. Therefore, formulated diet is required to establish sustainable lobster aquaculture. Concerning this issue, this study was carried out to assess the growth and survival of spiny lobster Panulirus homarus fed fresh food and formulated diet. The study was conducted in a completely randomized design (CRD) with 3 groups of treatments and 3 replicates for each treatment. Feeding experiment was performed in a flow-through water system using 9 fibre tanks, 4000 L in volume. Each tank was stocked with 40 lobsters, with initial weight of 78.08 ± 0.22 g. Each group of lobsters was fed with fresh food (A), formulated diet (pellet) (B) and a combination of pellet and fresh food (C). The fresh food was a mixture of fish, crabs, shrimp and small mussel (3:1:1:1). Feeding experiment was done for 15 weeks. Results of the experiment showed that the highest specific growth rate of lobster was obtained from lobsters fed with the combination of pellet and fresh food (0.52 ± 0.02 %/day) and the lowest growth was resulted from lobsters fed formulated diet (0.16 ± 0.03 %/day). In contrast, the highest survival was achieved in lobsters fed formulated diet (51.67%). Whereas the lowest survival was found in lobsters fed fresh food (10%). This study indicated that good growth of lobster was resulted from feeding with formulated diet combined with fresh food, while good survival was supported by feeding with formulated diet.

Fish hydrolysate contains hydrolyzed protein which comprised of peptides and free amino acids that are easily digested and absorbed by fish. This study aimed to determine the effectiveness of fish hydrolysate in improving feed efficiency and growth of coral trout. Five experimental diets were formulated to contain 47% protein and 12% lipid with different sources and levels of fish hydrolysate. Diet-1 and diet-2 were added with crustacean hydrolysate at 2% and 4% levels, respectively. Whereas diet-3 and diet-4 were supplemented with 2% and 4% of tuna hydrolysate. Diet-5 was formulated without fish hydrolysate supplementation (control). The diets were prepared as dry pellet with 3 mm diameter. Coral trout juveniles were produced in the hatchery of Institute for Mariculture Research and Fisheries Extension (IMRAFE), Gondol-Bali with the average body weight of 7.6 ± 0.9 g. The juveniles were maintained in 15 fiber tanks, 400L in volume, with a density of 40 fish/tank. The experiment was designed with a completely randomized design (CRD) with 5 treatments (different diets) and 3 replications for each treatment. The fish were fed the experimantal diets three times every day at satiation level for 84 days of feeding experiment. Results of the experiment showed that increased supplementation of fish hydrolysate from 2% to 4% in diet tended to improve fish growth performance. However, the best growth performance, in term of weight gain (WG) and specific growth rate (SGR), was obtained in fish fed diet supplemented with 4% crustacean hydrolysate. This growth performance was significantly higher than of the other treatments (P<0.05). The best feed efficiency (FE) and protein efficiency ratio (PER) were also obtained in fish fed diet supplemented with 4% crustacean hydrolysate. The results of the present study showed that supplementation of 4% crustacean hydrolysate in diet effectively improved the growth and feed efficiency of coral trout.

Abstract. The use of snakehead fish (Channa striata) collagen in cream is an alternative toreduce fish wastes. It has been proved that snakehead fish (Channa striata) contain collagen.This research aims to study the burn wound healing effectivity of bone’s collagen fromsnakehead fish (Channa striata) that is formulated into a cream. This study used theexperimental method with the bone of snakehead fish (Channa striata) as a sample. This studyconsisted of 4 (four) such as isolation of collagen from snakehead fish's (Channa striata) bone,characterization of collagen by analyzing moisture, ash, protein, fat and functional groupanalysis with FTIR, formulation, and evaluations of cream such as organoleptic test,homogeneity test, pH measurement, stability test, and irritation test, and burn wound healingtest in male white rats (Rattus norvegicus) and the result is analyzed using SPSS 22.0 Free trialand One Way ANOVA and Post-Hoc Tukey HSD. The results showed that fish collagen couldbe isolated from snakehead fish (Channa striata) and the yield obtained is 33.3%. The results ofcollagen characterization and evaluation test met the collagen standard requirements. Results ofthe burn wound healing test on male white rats (Rattus norvegicus) showed that K1 burn woundrecovered on day 21, K2 on day 12, K3 on day 15, K4 on day 18, and K5 on day 18.Measurement of burn wound diameter on day 21 showed K1 = 1.20 cm, K2 = 0.15 cm, K3 =0.10 cm, K4 = 0.45 cm, K5 = 0.40 cm. The results of the statistical analysis of burn wounddiameter showed a significant difference p = 0,000 (p <0.05) between each group. K3 showedthe reduction in the burn wound diameter is faster and the smallest, it can be concluded that theoptimal dose of snakehead fish bone’s collagen cream is a concentration of 3%.

Lobster pasir, Panulirus homarus adalah salah satu komoditas perikanan yang memiliki nilai ekonomis tinggi, baik di pasar lokal maupun internasional. Akan tetapi, dalam usaha pembesaran lobster terdapat hambatan yaitu tingginya mortalitas yang disebabkan oleh infeksi penyakit. Oleh karena itu, dilakukan penelitian ini dengan tujuan untuk melakukan investigasi penyakit pada pembesaran lobster pasir P. homarus di tiga lokasi karamba jaring apung (KJA). Metode penelitian meliputi pengambilan sampel lobster, pengamatan parasit, isolasi bakteri dan jamur, serta deteksi Milky Hemolymph Disease of Spiny Lobster (MHD-SL) melalui analisis Polymerase Chain Reaction (PCR). Lokasi pengambilan sampel meliputi Lombok (NTB), Pangandaran (Jabar) dan teluk Pegametan (Bali) dengan sampel lobster masing-masing sebanyak 5 ekor. Tiga ekor lobster dari Pangandaran terinfeksi parasit Octolasmis sp. yang menginfeksi terutama pada lamela insang. Satu sampel lobster dari Lombok terinfeksi jamur Fusarium sp. yang merupakan penyebab penyakit black gill disease dengan gejala insang yang menghitam. Dua lobster dari Lombok dan 1 lobster dari Pegametan terinfeksi oleh MHD-SL yang ditandai dengan hemolimfa berwarna putih susu.