Amphotericin B (AmB) is a polyene macrolide antibiotic that has antifungal action against the broadest range of fungal diseases as well as in some protozoan infections. There are currently no significant issues with resistance to AmB, and it is still in use today. The development of many innovative AmB formulations as lipid formulations and polymeric nanoparticles utilising UV-VIS spectrophotometry and HPLC technique has been discussed in the literature, however specifics of peak characteristics and validation data are not provided. The goal of this study was to provide an effective approach for validating AmB and to gather reliable, accurate, and consistent data on linearity, detection limit, quantification limit, accuracy, and precision using a UV-VIS spectrophotometer. The method gives a good linearity with regression of y = 0.1471x + 0.0489 (R2 = 0.9989) at 408 nm. The repeatability (inter-day) and intermediate precision (intra-day) precision studies revealed that the method is precise and reliable where all the RSD values were ˂2%. Method accuracy showed % accuracy value for all the three concentration levels ranged from 91.144 % to 101.994 % that a small change in the concentration of the drug could be accurately determined with high accuracy. Limit of detection and limit of quantification were 0.0295 (μg/ml) and 0.0895 (μg/ml) respectively. The suggested approach is straightforward and may be considered a strategy that is routinely practicable for estimating AmB.
Nanomaterials are emerging as an innovative and efficient instrument for the transport and cellular translocation of therapeutic compounds, namely, biopharmaceuticals. The use of nanoparticles as drug carriers in the healthcare industry, for instance, in several applications, with drug delivery being among the most significant. These nanomaterials are adaptable and have qualities that are good for delivering compounds that are biologically active. The creation of different nanoparticles as a drug delivery system has led to the development of a variety of nanoparticulate drug delivery systems to reduce toxicity, reduce the dosing system, and increase the viability of the drug. The objectives of this review are to provide an overview of the recent advances in nanotechnology in Antifungal treatment and to overcome the problems associated with antifungal agents.
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