The accuracy of state-of-the-art global barotropic tide models is assessed using bottom pressure data, coastal tide gauges, satellite altimetry, various geodetic data on Antarctic ice shelves, and independent tracked satellite orbit perturbations. Tide models under review include empirical, purely hydrodynamic ("forward"), and assimilative dynamical, i.e., constrained by observations. Ten dominant tidal constituents in the diurnal, semidiurnal, and quarter-diurnal bands are considered. Since the last major model comparison project in 1997, models have improved markedly, especially in shallow-water regions and also in the deep ocean. The root-sum-square differences between tide observations and the best models for eight major constituents are approximately 0.9, 5.0, and 6.5 cm for pelagic, shelf, and coastal conditions, respectively. Large intermodel discrepancies occur in high latitudes, but testing in those regions is impeded by the paucity of high-quality in situ tide records. Long-wavelength components of models tested by analyzing satellite laser ranging measurements suggest that several models are comparably accurate for use in precise orbit determination, but analyses of GRACE intersatellite ranging data show that all models are still imperfect on basin and subbasin scales, especially near Antarctica. For the M 2 constituent, errors in purely hydrodynamic models are now almost comparable to the 1980-era Schwiderski empirical solution, indicating marked advancement in dynamical modeling. Assessing model accuracy using tidal currents remains problematic owing to uncertainties in in situ current meter estimates and the inability to isolate the barotropic mode. Velocity tests against both acoustic tomography and current meters do confirm that assimilative models perform better than purely hydrodynamic models.

There are a number of leukemogenic protein-tyrosine kinases (PTKs) associated with leukemic transformation. Although each is linked with a specific disease their functional activity poses the question whether they have a degree of commonality in their effects upon target cells. Exon array analysis of the effects of six leukemogenic PTKs (BCR/ABL, TEL/PDGFR␤, FIP1/PDGFR␣, D816V KIT, NPM/ALK, and FLT3ITD) revealed few common effects on the transcriptome. It is apparent, however, that proteome changes are not directly governed by transcriptome changes. Therefore, we assessed and used a new generation of iTRAQ tagging, enabling eight-channel relative quantification discovery proteomics, to analyze the effects of these six leukemogenic PTKs. Again these were found to have disparate effects on the proteome with few common targets. BCR/ABL had the greatest effect on the proteome and had more effects in common with FIP1/PDGFR␣. The proteomic effects of the four type III receptor kinases were relatively remotely related. The only protein commonly affected was eosinophil-associated ribonuclease 7. Five of six PTKs affected the motility-related proteins CAPG and vimentin, although this did not correspond to changes in motility. However, correlation of the proteomics data with that from the exon microarray not only showed poor levels of correlation between transcript and protein levels but also revealed alternative patterns of regulation of the CAPG protein by different oncogenes, illustrating the utility of such a combined approach. Molecular & Cellular Proteomics 7: 853-863, 2008.

Stratified flow over topography is studied, with oceanic applications in mind. A model is developed for a fluid with arbitrary vertical stratification and a free surface, flowing over three-dimensional topography of arbitrary size and steepness, with background rotation, in the linear hydrostatic regime. The model uses an expansion of the flow fields in terms of a set of basis functions, which efficiently capture the vertical dependence of the flow. The horizontal structure may then be found by solving a set of coupled partial differential equations in two horizontal directions and time, subject to simple boundary conditions. In some cases, these equations may be solved analytically, but, in general, simple numerical procedures are required. Using this formulation, the internal tide generated by a time-periodic barotropic tidal flow over a continental shelf and slope is calculated in various idealized configurations. The topography and fluid motion are taken to be independent of one coordinate direction and the fluid to be either two-layer or uniformly stratified. For the two-layer case, expressions for the shoreward and oceanward energy fluxes associated with the internal tide are derived. For the uniformly stratified case, it is studied numerically how the accuracy of the solutions depends upon the number of basis functions used, and it is shown that good solutions and energy flux estimates can often be obtained with only a few basis functions. In both cases, the results show that the position of the coastline, through its effect on the form of the barotropic tide, significantly influences the strength of the internal tide generation.

Diurnal and semidiurnal ocean tides are calculated for both the present day and the Last Glacial Maximum. A numerical model with complete global coverage and enhanced resolution at high latitudes is used including the physics of self-attraction and loading and internal tide drag. Modeled present-day tidal amplitudes are overestimated at the standard resolution, but the error decreases as the resolution increases. It is argued that such results, which can be improved in the future using higher-resolution simulations, are preferable to those obtained by artificial enhancement of dissipative processes. For simulations at the Last Glacial Maximum a new version of the ICE-5G topographic reconstruction is used along with density stratification determined from coupled atmosphere-ocean climate simulations. The model predicts a significant amplification of tides around the Arctic and Antarctic coastlines, and these changes are interpreted in terms of Kelvin wave dynamics with the aid of an exact analytical solution for propagation around a polar continent or basin. These polar tides are shown to be highly sensitive to the assumed location of the grounding lines of coastal ice sheets, and the way in which this may contribute to an interaction between tides and climate change is discussed. Globally, the picture is one of energized semidiurnal tides at the Last Glacial Maximum, with an increase in tidal dissipation from present-day values, the dominant energy sink being the conversion to internal waves.