Toxoplasma gondii is a unique intracellular parasite, which infect a large proportion of the world population, but uncommonly causes clinically significant disease. The present study was performed to estimate the prevalence of toxoplasmosis in 400 apparently healthy blood donor males, their ages were between 18 and 57 years using enzyme linked immunosorbent assay, and to examine the effects of infection on total testosterone, free testosterone and follicle stimulating hormone (FSH) levels in their sera. Seroprevalence showed 10 (2.5 %) and 121 (30 %) of them had IgM and IgG antibodies respectively. Both acute and chronic toxoplasmosis in males recorded higher significant (P \ 0.05) mean concentration for total and free testosterone hormone, they were 12.188 ± 0.73, 7.837 ± 0.52 ng/ ml and 44.121 ± 1.76, 27.984 ± 0.94 pg/ml respectively. The mean concentration of FSH revealed non-significant (P \ 0.05) differences in both disease activities, they were 6.41 ± 0.47 and 6.515 ± 0.51 IU/ml respectively.