Amplification technology showed that HPV is common among women in Riyadh, Saudi Arabia, with a strong association between HPV infection and cytological changes. HPV-16 was the most frequent genotype but had a low prevalence of cervical cancer.

A rapid and sensitive TLC method was developed and validated for the analysis of salbutamol and identification of its related impurities compounds. Spectro-densitometric scanning-integration was performed at an absorbance wave length of 254 nm. To justify the suitability of the proposed method, accuracy, precision and recovery studies were performed at three selected concentrations levels. The recovery data reveals that the RSD for intra-day and inter-day analysis were found to be 4.86 and 1.23%, respectively. A TLC plastic plate precoated with silica gel was used as the stationary phase. The solvent system was acetonitrile : methanol : ammonium 2099 ORDER REPRINTS hydroxide (10 : 85 : 5 v/v/v) gave a dense and compact spots of salbutamol and related impurities compounds namely: isopropyl salbutamol, desoxy salbutamol base, salbutamol ketone hydrochloride and 5-formyl saligenin salbutamol with a Rf values of 0.1, 0.26, 0.35, 0.54 and 0.75 respectively. The calibration plots exhibited good linear relationship (r ¼ 0.9996) over a concentration range of 5-25 mg/ml. Statical analysis proved that the proposed method is accurate and reproducible. The method is stability indicating and being economical can be employed for the routine analysis of the bulk material of salbutamol and its pharmaceutical tablets formulations.

No abstract

A quick and sensitive reversed-phase HPLC method has been developed for the analysis of 2'-beta -fluoro-2',3'-dideoxy adenosine (F-ddA), the acid-stable anti-AIDS drug, and its metabolite 2'-fluoro-2',3'-dideoxy inosine (F-ddI) in human plasma using polyfluorinated stationary phase column (Fluo fix, 15 cm, 4.0 mm i.d., 5 microm particle size). The mobile phase consisted of ammonium phosphate buffer solution (10 mM) adjusted with phosphoric acid 85% to pH 6.8:dimethyl formamide (97:3, v/v). F-ddA and F-ddI were monitored by UV-visible detector at 258 and 247 nm, respectively. The recoveries of F-ddA and F-ddI from plasma using a C(18) solid-phase extraction cartridge were 99.2% and 99.7%, respectively.

No abstract